| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAP3 |
| Uniprot No | P50453 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-376aa |
| 氨基酸序列 | METLSNASGTFAIRLLKILCQDNPSHNVFCSPVSISSALAMVLLGAKGNTATQMAQALSLNTEEDIHRAFQSLLTEVNKAGTQYLLRTANRLFGEKTCQFLSTFKESCLQFYHAELKELSFIRAAEESRKHINTWVSKKTEGKIEELLPGSSIDAETRLVLVNAIYFKGKWNEPFDETYTREMPFKINQEEQRPVQMMYQEATFKLAHVGEVRAQLLELPYARKELSLLVLLPDDGVELSTVEKSLTFEKLTAWTKPDCMKSTEVEVLLPKFKLQEDYDMESVLRHLGIVDAFQQGKADLSAMSAERDLCLSKFVHKSFVEVNEEGTEAAAASSCFVVAECCMESGPRFCADHPFLFFIRHNRANSILFCGRFSSP |
| 预测分子量 | 69.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAP3重组蛋白的3篇示例文献(内容为虚构，仅作格式参考)：
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1. **文献名称**：Cloning and Expression of Recombinant CAP3 in Escherichia coli
**作者**：Smith A, et al.
**摘要**：研究报道了通过PCR扩增CAP3基因，并将其克隆至pET载体系统，利用大肠杆菌BL21(DE3)实现高效可溶性表达。纯化的重组CAP3在体外展示了DNA结合活性，为后续功能研究奠定基础。
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2. **文献名称**：Structural Analysis of CAP3 Recombinant Protein in DNA Repair Complex
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析了重组CAP3蛋白的晶体结构，揭示了其与DNA末端结合的关键结构域，并证明CAP3在非同源末端连接(NHEJ)修复通路中的重要作用。
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3. **文献名称**：Functional Characterization of CAP3 in Viral Genome Packaging
**作者**：Chen L, et al.
**摘要**：研究利用杆状病毒表达系统制备重组CAP3蛋白，发现其与疱疹病毒基因组包装信号特异性互作，提示CAP3可能作为抗病毒治疗的潜在靶点。
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(注：以上文献为模拟内容，实际研究中请通过PubMed或Web of Science检索真实文献。)
**Background of CAP3 Recombinant Protein**
CAP3 (Chromosome Assembly Protein 3), also known as CSE1L (Cellular Apoptosis Susceptibility Protein Export 1-Like), is a multifunctional protein involved in nucleocytoplasmic transport, cell cycle regulation, and apoptosis. It was initially identified in yeast as a member of the CAP (Chromosome Alignment Protein) complex, which plays a role in chromosome segregation during mitosis. In mammalian systems, CAP3 homologs are evolutionarily conserved and share structural similarities with yeast counterparts, including coiled-coil domains critical for protein-protein interactions.
CAP3 is a key component of the nuclear export machinery, facilitating the transport of proteins and RNAs between the nucleus and cytoplasm. It interacts with Ran GTPase and exportin proteins to mediate cargo recognition and release. Notably, CAP3 is essential for the export of importin-α, a regulator of nuclear import, ensuring the dynamic balance of nucleocytoplasmic trafficking. Beyond transport, CAP3 participates in mitotic spindle assembly, chromosome alignment, and checkpoint signaling, linking it to genomic stability and cell division fidelity.
Recombinant CAP3 protein is engineered for structural and functional studies. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains native folding and activity. Its recombinant form enables biochemical assays, interaction mapping (e.g., with Ran or exportins), and exploration of its roles in cancer biology, where CAP3 overexpression correlates with tumor progression and drug resistance. Additionally, recombinant CAP3 serves as a tool to study mutations affecting transport pathways or mitotic errors. Purified with tags (e.g., His-tag), it supports antibody development and high-throughput screening for therapeutic targeting. Overall, CAP3 recombinant protein is vital for dissecting its dual roles in cellular logistics and disease mechanisms.
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