关键信息
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基因名
PLA2G12
- 应用
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别名
PLA2G12；PLA2G12；Group XIIA secretory phospholipase A2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BZM1
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表达区间
23-185aa
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氨基酸序列
QEQAQTTDWRATLKTIRNGVHKIDTYLNAALDLLGGEDGLCQYKCSDGSKPFPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICRDVQKTLGLTQHVQACETTVELLFDSVIHLGCKPYLDSQRAACRCHYEE
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分子量
20.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PLA2G12, or Phospholipase A2 Group XII, is a member of the phospholipase A2 enzyme family, which plays a critical role in lipid metabolism and inflammatory responses. This enzyme is known for its ability to hydrolyze phospholipids, leading to the release of arachidonic acid and lysolipids, key mediators in various biological processes such as cell signaling, inflammation, and cellular membrane dynamics. Research has highlighted PLA2G12's involvement in pathophysiological conditions, including cardiovascular diseases and cancer, where its enzymatic activity may modulate tumor progression and metastasis. The recombinant production of PLA2G12 allows for detailed functional studies and potential therapeutic applications, including the development of inhibitors or modulators that could influence its activity in disease contexts. Additionally, the study of this protein could provide insights into the enzymatic mechanisms underlying lipid signaling pathways, contributing to a greater understanding of cell biology and the development of targeted interventions in associated diseases. Overall, PLA2G12 represents a significant focus of research due to its biological relevance and potential as a therapeutic target, calling for ongoing investigations into its structure, function, and clinical implications.
