关键信息
-
基因名
PPM1F
- 应用
-
别名
PPM1F;KIAA0015;POPX2;Protein phosphatase 1F
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P49593
-
表达区间
1-454aa
-
氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMSSGAPQKSSPMASGAEETPGFLDTLL QDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRKAPPPLAA ALAHEAVSQLLQTDLSEFRKLPREEEEEEEDDDEEEKAPVTLLDAQSLAQ SFFNRLWEVAGQWQKQVPLAARASQRQWLVSIHAIRNTRRKMEDRHVSLP SFNQLFGLSDPVNRAYFAVFDGHGGVDAARYAAVHVHTNAARQPELPTDP EGALREAFRRTDQMFLRKAKRERLQSGTTGVCALIAGATLHVAWLGDSQV ILVQQGQVVKLMEPHRPERQDEKARIEALGGFVSHMDCWRVNGTLAVSRA IGDVFQKPYVSGEADAASRALTGSEDYLLLACDGFFDVVPHQEVVGLVQS HLTRQQGSGLRVAEELVAAARERGSHDNITVMVVFLRDPQELLEGGNQGE GDPQAEGRRQDLPSSLPEPETQAPPRS
-
分子量
52 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PPM1F (Protein Phosphatase, Mg2+/Mn2+ Dependent 1F) is a serine/threonine phosphatase that plays a crucial role in the regulation of various cellular processes, including cell cycle progression, apoptosis, and response to stress. The enzyme is known to dephosphorylate target proteins, thereby modulating their activity and function. Research has shown that PPM1F is involved in several signaling pathways, making it a significant player in cellular signaling networks. Abnormal expression or activity of PPM1F has been associated with various diseases, including cancer, where it can influence tumor development and progression. By studying recombinant PPM1F, researchers aim to better understand its biochemical properties and interactions with substrates, providing insights into its role in health and disease. Furthermore, the investigation of PPM1F's mechanism of action may uncover potential therapeutic targets for diseases associated with dysregulated phosphatase activity. Recombinant forms of this protein facilitate the study of its enzymatic activity, substrate specificity, and regulatory mechanisms, enabling the identification of novel pathways and the development of intervention strategies. This research also contributes to the broader understanding of phosphatase functions in cellular homeostasis and the intricate balance of phosphorylation/dephosphorylation processes critical for normal cellular functioning.
