| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAA4 |
| Uniprot No | P35542 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-130aa |
| 氨基酸序列 | ESWRSFFKEALQGVGDMGRAYWDIMISNHQNSNRYLYARGNYDAAQRGPG GVWAAKLISRSRVYLQGLIDYYLFGNSSTVLEDSKSNEKAEEWGRSGKDP DRFRPDGLPKKY |
| 预测分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAA4重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Serum Amyloid A4 is a Pro-Inflammatory Cytokine that Enhances Vascular Cell Adhesion"*
**作者**：Liang, T.S. et al.
**摘要**：本研究通过重组表达人源SAA4蛋白，分析了其在血管内皮细胞中的促炎作用。实验表明，重组SAA4能显著促进单核细胞与内皮细胞的黏附，并激活NF-κB信号通路，提示其在动脉粥样硬化中的潜在作用。
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2. **文献名称**：*"Structural and Functional Characterization of Recombinant Human SAA4"*
**作者**：De Beer, M.C. et al.
**摘要**：文章报道了通过大肠杆菌系统重组表达并纯化人源SAA4蛋白的方法，解析了其二级结构特征。研究发现，重组SAA4与高密度脂蛋白(HDL)结合能力较弱，可能参与脂质代谢调控，区别于其他SAA家族成员。
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3. **文献名称**：*"Role of SAA4 in Amyloidogenesis: Insights from Recombinant Protein Studies"*
**作者**：Yamada, T. et al.
**摘要**：通过重组SAA4蛋白的体外实验，研究发现其不易形成淀粉样纤维，但可与SAA1协同促进纤维沉积，为遗传性淀粉样变性的发病机制提供了新视角。
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如需进一步文献或具体研究细节，建议在PubMed或Web of Science中以“recombinant SAA4 protein”或“SAA4 expression”为关键词检索。
**Background of SAA4 Recombinant Protein**
Serum amyloid A4 (SAA4) is a member of the serum amyloid A (SAA) family, a group of highly conserved apolipoproteins involved in inflammation, lipid metabolism, and immune regulation. While most SAA isoforms, such as SAA1 and SAA2. are acute-phase reactants synthesized predominantly in the liver during inflammation, SAA4 is considered a constitutively expressed "housekeeping" isoform. It is produced at baseline levels in various tissues, including the liver, intestine, and macrophages, and its expression remains relatively stable during inflammatory responses.
Structurally, SAA4 shares a conserved N-terminal domain with other SAAs, critical for lipid binding and amyloid fibril formation, but differs in its C-terminal region due to unique glycosylation patterns. This post-translational modification distinguishes SAA4 as the only glycosylated SAA isoform, influencing its stability, solubility, and functional interactions.
Functionally, SAA4 is implicated in high-density lipoprotein (HDL) metabolism, facilitating cholesterol transport and cellular lipid uptake. It also exhibits immunomodulatory properties, though its role in inflammation remains less defined compared to acute-phase SAAs. Emerging studies suggest SAA4 may serve as a biomarker for metabolic disorders, cardiovascular diseases, and certain cancers due to its altered expression in pathological conditions.
Recombinant SAA4 protein is generated using expression systems like *E. coli* or mammalian cells to ensure proper folding and glycosylation. Its production enables mechanistic studies on SAA4-specific functions, therapeutic development, and diagnostic applications. Researchers utilize recombinant SAA4 to explore its dual role in homeostasis and disease, bridging gaps in understanding the SAA family's diverse biological impacts.
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