| 纯度 | >95% |
| 种属 | Mouse |
| 靶点 | 聽Osteopontin/OPN |
| Uniprot No | Q547B5 |
| 内毒素 | ＜0.1EU/ug |
| 表达宿主 | 293F |
| 表达区间 | Leu17-Asn294 (Glu99Gly) |
| 活性数据 | Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 105 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hourincubation at 37 °C. The ED50 forthis effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold. |
| 分子量 | 30 kDa & 65 kDa |
| 蛋白标签 | C-His |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Recombinant Mouse Osteopontin/OPN Protein (Active) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than >95%
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