| 纯度 | >95% |
| 种属 | Human |
| 靶点 | Frizzled-1 |
| Uniprot No | Q9UP38 |
| 内毒素 | ＜0.1EU/ug |
| 表达宿主 | 293F |
| 表达区间 | 73- 253 aa |
| 活性数据 | Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-1/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-1/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20- 80 ng/mL. Optimal concentrations should be determined by each laboratory for each application. |
| 分子量 | 59- 66 kDa |
| 蛋白标签 | C-hFc&His tag |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Recombinant Human Frizzled-1 Protein (Active) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than >95%
×
