| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTSC |
| Uniprot No | P53634-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 氨基酸序列 | MGAGPSLLLA ALLLLLSGDG AVRCDTPANC TYLDLLGTWV FQVGSSGSQR DVNCSVMGPQ EKKVVVYLQK LDTAYDDLGN SGHFTIIYNQ GFEIVLNDYK WFAFFKYKEE GSKVTTYCNE TMTGWVHDVL GRNWACFTGK KVGTASENVY VNIAHLKNSQ EKYSNRLYKY DHNFVKAINA IQKSWTATTY MEYETLTLGD MIRRSGGHSR KIPRPKPAPL TAEIQQKILH LPTSWDWRNV HGINFVSPVR NQASCGSCYS FASMGMLEAR IRILTNNSQT PILSPQEVVS CSQYAQGCEG GFPYLIAGKY AQDFGLVEEA CFPYTGTDSP CKMKEDCFRY YSSEYHYVGG FYGGCNEALM KLELVHHGPM AVAFEVYDDF LHYKKGIYHH TGLRDPFNPF ELTNHAVLLV GYGTDSASGM DYWIVKNSWG TGWGENGYFR IRRGTDECAI ESIAVAATPI PKL |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CTSC(组织蛋白酶C)重组蛋白相关的文献概览：
1. **文献名称**: *"Recombinant human cathepsin C (dipeptidyl peptidase I) corrects the disease phenotype in a model of Papillon-Lefèvre syndrome"*
**作者**: Korkmaz, B., et al.
**摘要**: 研究通过重组表达人源CTSC蛋白，验证其在体外对Papillon-Lefèvre综合征(CTSC基因缺陷)细胞模型的治疗潜力，证明其可恢复中性粒细胞的丝氨酸蛋白酶活性。
2. **文献名称**: *"Expression and characterization of recombinant cathepsin C: Key enzyme in granzyme B activation"*
**作者**: Pham, C.T.N., et al.
**摘要**: 报道利用昆虫细胞系统高效表达重组CTSC蛋白，分析其酶动力学特性，揭示其在颗粒酶B激活中的关键作用，为免疫相关疾病研究提供工具。
3. **文献名称**: *"Structural insights into the activation mechanism of cathepsin C by recombinant expression and crystallography"*
**作者**: Guay, D., et al.
**摘要**: 通过重组CTSC蛋白的晶体结构解析，阐明其酶原激活的分子机制，为开发CTSC抑制剂治疗慢性炎症性疾病提供结构基础。
*注：以上文献信息为示例性质，具体内容需根据实际文献调整。建议通过PubMed或Google Scholar以关键词“CTSC recombinant protein”或“Cathepsin C expression”检索最新研究。*
Cathepsin C (CTSC), also known as dipeptidyl peptidase I, is a lysosomal cysteine protease belonging to the papain superfamily. It plays a critical role in activating serine proteases in immune cells, including neutrophil elastase and granzymes, by cleaving N-terminal dipeptides from precursor proteins. Structurally, CTSC exists as a tetramer composed of two heavy and two light chains, requiring chloride ions for optimal enzymatic activity. Its gene is located on chromosome 11q14.1-q14.3 in humans.
CTSC gained attention due to its association with genetic disorders like Papillon-Lefèvre syndrome and Haim-Munk syndrome, characterized by severe periodontal disease and palmoplantar hyperkeratosis. Mutations in the CTSC gene lead to loss of enzymatic function, impairing immune cell protease activation and epithelial homeostasis. Beyond hereditary diseases, CTSC overexpression has been implicated in chronic inflammation, rheumatoid arthritis, and cancer progression, making it a potential therapeutic target.
Recombinant CTSC proteins are typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) or insect cell cultures to ensure proper post-translational modifications. These engineered proteins enable detailed structural studies, inhibitor screening, and mechanism-based drug development. Recent research focuses on designing CTSC-specific inhibitors for inflammatory conditions while preserving related protease functions. In diagnostic applications, recombinant CTSC serves as an antigen for antibody development and biomarker analysis. Current challenges include understanding its dual roles in immune regulation and tissue destruction, as well as optimizing pharmacological modulation for clinical translation.
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