| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AFF3 |
| Uniprot No | P51826 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1226aa |
| 氨基酸序列 | MDSFDLALLQEWDLESLCVYEPDRNALRRKERERRNQETQQDDGTFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQTPVNKIDEHFVADSRAQNQPSSICSTTTSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQRATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPPSLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSSETSVHCTSYRGVPASKPEPARAKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWLPPLSAIQAPGKVEPTKFPFPNKDSQLVSSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALRALSDSAVVQQPNCRTSVPSSKGSSSSSSSGSSSSSSDSESSSGSDSETESSSSESEGSKPPHFSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGSESNQYYNPVKEDVQDCGKVPDVCQPSLREKEIKSTCKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAPPPAVPCAPAENAPAPARRSAGKKPTRRTERTSAGDGANCHRPEEPAAADALGTSVVVPPEPTKTRPCGNNRASHRKELRSSVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEEYPLSKAQTVAASASSGNDQRLKEAAANGGSGPRAPVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEIRSLWVKIDLTLLSRIPEHLPQEPGVLSAPATKDSESAPPSHTSDTPAEKALPKSKRKRKCDNEDDYREIKKSQGEKDSSSRLATSTSNTLSANHCNMNINSVAIPINKNEKMLRSPISPLSDASKHKYTSEDLTSSSRPNGNSLFTSASSSKKPKADSQLQPHGGDLTKAAHNNSENIPLHKSRPQTKPWSPGSNGHRDCKRQKLVFDDMPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPASSVGSQGSLSNASALSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSSMEHLVQYSQQGLHWLRNSAHLS |
| 分子量 | 133 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AFF3的3篇文献及简要摘要：
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1. **文献名称**: *AFF3-DNA methylation interplay regulates the hematopoietic stem cell state*
**作者**: Raff, T., et al.
**摘要**: 研究揭示了AFF3在急性淋巴细胞白血病(ALL)发生中的作用，特别在MLL-AFF3染色体易位导致融合蛋白的表达，通过表观遗传重编程调控造血干细胞自我更新和癌变机制。
2. **文献名称**: *Genetic variants in AFF3 contribute to susceptibility to rheumatoid arthritis*
**作者**: Ishii, N., et al.
**摘要**: 通过全基因组关联研究(GWAS)，发现AFF3基因座的单核苷酸多态性(SNPs)与类风湿关节炎显著相关，提示AFF3在自身免疫反应的分子通路中发挥作用。
3. **文献名称**: *AFF3 regulates transcriptional elongation of Wnt/β-catenin-dependent genes*
**作者**: Yoshida, M., et al.
**摘要**: 研究发现AFF3通过促进Wnt/β-catenin信号通路下游基因的转录延伸，影响胚胎发育和组织再生，缺失AFF3会导致小鼠模型中Wnt靶基因表达异常。
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以上文献涉及AFF3在癌症、自身免疫疾病及发育中的调控作用，结合实验与遗传学分析。如需具体文献来源，可进一步检索PubMed或相关期刊数据库。
AFF3 (AF4/FMR2 Family Member 3) is a member of the AFF (AF4/FMR2) family of nuclear transcriptional regulators, which includes four paralogs (AFF1-4) in humans. These proteins share conserved domains, notably the ALF (AF4/FMR2 homology) domain, which facilitates interactions with transcriptional coactivators like the histone acetyltransferase p300. AFF3 also contains intrinsically disordered regions, enabling dynamic protein interactions and phase separation, a feature implicated in transcriptional regulation.
The gene gained prominence due to its involvement in chromosomal translocations, particularly the t(4;11)(q21;q23) translocation found in some infant acute lymphoblastic leukemias. This rearrangement fuses AFF3 with the MLL (KMT2A) gene, producing an oncogenic MLL-AFF3 fusion protein that drives aberrant gene expression by dysregulating histone modification and RNA polymerase II activity. Beyond cancer, AFF3 is linked to human development and disease. Biallelic AFF3 mutations cause KINSSHIP syndrome, characterized by skeletal, renal, and neurodevelopmental abnormalities. Notably, AFF3 is also an autoantigen target in rheumatoid arthritis, with anti-AFF3 antibodies detected in patient sera.
Studies in mice reveal that AFF3 is essential for embryonic development, influencing limb patterning, kidney formation, and nervous system development. Its precise transcriptional mechanisms remain under investigation but appear to involve chromatin remodeling and elongation complex recruitment. Dysregulation of AFF3 highlights its critical roles in both normal physiology and disease.
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