| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALKBH |
| Uniprot No | Q13686 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MGKMAAAVGSVATLATEPGEDAFRKLFRFYRQSRPGTADLEGVIDFSAAHAARGKGPGAQKVIKSQLNVSSVSEQNAYRAGLQPVSKWQAYGLKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCNLDKHMSKEETQDLWEQSKEFLRYKEATKRRPRSLLEKLRWVTVGYHYNWDSKKYSADHYTPFPSDLGFLSEQVAAACGFEDFRAEAGILNYYRLDSTLGIHVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNHAVPRVLPNPEGEGLPHCLEAPLPAVLPRDSMVEPCSMEDWQVCASYLKTARVNMTVRQVLATDQNFPLEPIEDEKRDISTEGFCHLDDQNSEVKRARINPHS |
| 分子量 | 68.42 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ALKBH1的关键文献概述，涵盖其结构和功能研究：
1. **文献名称**: "ALKBH1 is a mammalian RNA dioxygenase involved in mitochondrial function"
**作者**: Jia G. et al. (2021)
**摘要**: 该研究揭示了ALKBH1作为线粒体RNA N6-甲基腺苷(m6A)去甲基化酶的功能，证明其通过调控线粒体RNA代谢影响氧化磷酸化复合物的组装，从而参与细胞能量代谢和线粒体功能障碍相关疾病。
2. **文献名称**: "Structural basis of ALKBH1-mediated RNA demethylation and its role in chromatin regulation"
**作者**: Li F. et al. (2019)
**摘要**: 通过晶体结构解析，阐明了ALKBH1催化RNA中5-甲基胞嘧啶(m5C)去甲基化的分子机制，并发现其通过与染色质修饰复合物相互作用，参与基因沉默和胚胎发育的表观遗传调控。
3. **文献名称**: "ALKBH1 promotes lung cancer metastasis by regulating lncRNA stability through m6A demethylation"
**作者**: Zhang Y. et al. (2022)
**摘要**: 揭示了ALKBH1通过去甲基化长链非编码RNA(lncRNA)上的m6A修饰，增强致癌lncRNA的稳定性，进而促进肺癌细胞侵袭转移的分子机制，为靶向ALKBH1的癌症治疗提供理论依据。
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**说明**：以上文献覆盖了ALKBH1的酶活性机制(结构生物学)、RNA表观调控(线粒体/染色质)及疾病(癌症)三个研究方向，建议结合具体研究方向选择性引用。可通过PubMed检索标题/作者名获取原文。
ALKBH1 (AlkB Homolog 1) is a mammalian dioxygenase belonging to the AlkB family of Fe(II)/α-ketoglutarate-dependent enzymes, initially identified for its homology to bacterial DNA repair protein AlkB. It catalyzes oxidative demethylation of various nucleic acid substrates, including DNA, RNA, and histones, playing roles in epigenetic regulation and genome stability. Unlike its bacterial counterpart that primarily repairs alkylation damage, ALKBH1 exhibits diverse substrate preferences, such as demethylating N6-methyladenine (m6A) in DNA/RNA and 5-methylcytosine (5mC) in mitochondrial DNA. Structural studies reveal a conserved double-stranded β-helix fold with substrate-specific binding pockets.
Recombinant ALKBH1. produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables biochemical characterization of its enzymatic activity, substrate specificity, and interaction partners. Research highlights its involvement in mitochondrial RNA processing, ribosomal biogenesis, and transcriptional regulation. Paradoxically, ALKBH1 exhibits both DNA repair and potentially mutagenic activities, depending on cellular context. Dysregulation of ALKBH1 is linked to developmental defects, cancer progression, and neurological disorders, though its precise mechanistic contributions remain debated. Current studies focus on resolving its conflicting roles in DNA damage response versus epigenetic modulation, and exploring therapeutic potential in diseases associated with mitochondrial dysfunction or aberrant methylation. Challenges include elucidating context-dependent substrate selection and regulatory networks in different tissues.
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