| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DPP7 |
| Uniprot No | Q9UHL4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-492aa |
| 氨基酸序列 | APDPG FQERFFQQRL DHFNFERFGN KTFPQRFLVS DRFWVRGEGP IFFYTGNEGD VWAFANNSAF VAELAAERGA LLVFAEHRYY GKSLPFGAQS TQRGHTELLT VEQALADFAE LLRALRRDLG AQDAPAIAFG GSYGGMLSAY LRMKYPHLVA GALAASAPVL AVAGLGDSNQ FFRDVTADFE GQSPKCTQGV REAFRQIKDL FLQGAYDTVR WEFGTCQPLS DEKDLTQLFM FARNAFTVLA MMDYPYPTDF LGPLPANPVK VGCDRLLSEA QRITGLRALA GLVYNASGSE HCYDIYRLYH SCADPTGCGT GPDARAWDYQ ACTEINLTFA SNNVTDMFPD LPFTDELRQR YCLDTWGVWP RPDWLLTSFW GGDLRAASNI IFSNGNLDPW AGGGIRRNLS ASVIAVTIQG GAHHLDLRAS HPEDPASVVE ARKLEATIIG EWVKAARREQ QPALRGGPRL SL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DPP7重组蛋白的3篇示例文献(注：文献为虚构示例，仅供格式参考)：
1. **文献名称**: *"Heterologous expression and enzymatic characterization of human dipeptidyl peptidase 7 (DPP7) in Pichia pastoris*"
**作者**: Müller, A. et al.
**摘要**: 本研究利用毕赤酵母系统成功表达重组人源DPP7.通过亲和层析纯化获得高纯度蛋白，并验证其水解Gly-Pro-AMC底物的酶活性，揭示了DPP7在pH 7.5条件下的最适催化环境。
2. **文献名称**: *"Crystal structure of recombinant DPP7 from Bacillus subtilis and its substrate recognition mechanism*"
**作者**: Tanaka, K. et al.
**摘要**: 通过X射线晶体学解析枯草芽孢杆菌DPP7重组蛋白的三维结构，发现其活性位点对疏水性二肽底物的选择性，为设计特异性抑制剂提供结构基础。
3. **文献名称**: *"Functional analysis of DPP7 in macrophage proteostasis using CRISPR/Cas9 and recombinant protein rescue*"
**作者**: Chen, L. et al.
**摘要**: 结合基因编辑技术及重组DPP7蛋白回补实验，证实DPP7通过降解特定胞内二肽调控巨噬细胞的蛋白质稳态，影响炎症因子分泌。
如需真实文献，建议在PubMed或Web of Science中检索关键词“DPP7 recombinant”或“DPP7 expression”。
**Background of Recombinant DPP7 Protein**
Dipeptidyl peptidase 7 (DPP7), also known as dipeptidyl peptidase II or quiescent cell proline dipeptidase (QPP), is a serine protease belonging to the S9B subfamily of prolyl oligopeptidases. It catalyzes the cleavage of dipeptides from the N-terminus of peptides with a proline or alanine residue at the penultimate position. DPP7 is ubiquitously expressed in tissues, particularly in the kidney, liver, and immune cells, and is localized intracellularly, often in lysosomes or cytoplasmic compartments. Its enzymatic activity is linked to diverse physiological processes, including protein turnover, immune regulation, and cellular homeostasis.
Recombinant DPP7 protein is engineered through heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to enable functional and structural studies. Unlike other DPP family members (e.g., DPP4/CD26), DPP7 has distinct substrate specificity and is less characterized, though emerging research highlights its role in modulating bioactive peptides, chemokines, and inflammatory responses. Dysregulation of DPP7 has been implicated in immune disorders, cancer progression, and neurodegenerative diseases, making it a potential therapeutic target.
The production of recombinant DPP7 involves codon optimization, purification via affinity tags, and validation of enzymatic activity using fluorogenic substrates. Challenges include maintaining proper folding and post-translational modifications critical for activity. Current applications span drug discovery, biomarker research, and mechanistic studies of peptide metabolism. Advances in structural biology (e.g., X-ray crystallography) have begun elucidating DPP7’s catalytic mechanism, aiding inhibitor design. Despite its understudied status, DPP7’s unique enzymatic profile positions it as a promising focus for biomedical research.
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