| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | S100A5 |
| Uniprot No | P33763 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-92aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMETPLEKALTTMVTTFHKYSGREGSKLTLS RKELKELIKKELCLGEMKESSIDDLMKSLDKNSDQEIDFKEYSVFLTMLC MAYNDFFLEDNK |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于S100A5重组蛋白的3篇代表性文献，内容涵盖结构、功能及疾病关联研究：
1. **文献名称**：*Recombinant human S100A5 forms a dimer with distinct calcium-binding properties*
**作者**：Smith A, et al.
**摘要**：该研究利用大肠杆菌系统成功表达并纯化了重组人源S100A5蛋白，发现其在溶液中以二聚体形式存在，并通过圆二色谱和等温滴定量热法证实其具有独特的钙离子结合能力，暗示其在细胞内钙信号传导中的潜在作用。
2. **文献名称**：*S100A5 promotes tumor cell migration via interaction with RAGE: Insights from recombinant protein-based assays*
**作者**：Chen L, et al.
**摘要**：作者通过原核表达获得高纯度重组S100A5蛋白，发现其能与细胞表面受体RAGE结合，激活下游MAPK通路，促进乳腺癌细胞迁移。实验结果为S100A5在肿瘤转移中的机制提供了分子证据。
3. **文献名称**：*Structural characterization of recombinant S100A5 and its interaction with zinc ions*
**作者**：Wang Y, et al.
**摘要**：该研究通过核磁共振解析了重组S100A5的三维结构，首次揭示其锌离子结合位点位于EF-hand结构域附近，并发现锌结合显著增强其与靶蛋白的亲和力，提示金属离子协同调节S100A5的生物学功能。
注：以上文献为示例性内容，实际引用时需根据具体研究领域补充真实发表的论文信息(可通过PubMed/Google Scholar检索关键词"S100A5 recombinant protein"获取最新文献)。
S100A5 is a member of the S100 family of calcium-binding proteins, characterized by two EF-hand domains that enable calcium-dependent regulation of cellular processes. Encoded on human chromosome 1q21. S100A5 shares structural homology with other S100 proteins but exhibits unique expression patterns and functional roles. It is predominantly expressed in olfactory epithelium, specific neuronal populations, and select tissues like skin, lung, and reproductive organs, suggesting tissue-specific regulatory functions.
Functionally, S100A5 participates in intracellular calcium signaling and extracellular interactions with receptor for advanced glycation end products (RAGE) or other surface receptors. It modulates inflammatory responses by influencing proinflammatory cytokine release and neutrophil migration. In the nervous system, it regulates neuronal excitability and synaptic plasticity, potentially linked to olfactory signal transduction and neurodevelopmental processes. Aberrant S100A5 expression has been associated with pathological conditions, including respiratory diseases (e.g., asthma), neuroinflammatory disorders, and various cancers, where it may promote tumor progression through matrix metalloproteinase activation or angiogenesis.
Recombinant S100A5 protein, produced via prokaryotic (E. coli) or eukaryotic expression systems, retains native conformation and bioactivity when purified through affinity chromatography. Its recombinant form enables mechanistic studies of calcium-mediated signaling, structural analyses of target interactions, and therapeutic exploration. Researchers utilize it to investigate S100A5's dual roles as an intracellular calcium sensor and extracellular damage-associated molecular pattern (DAMP) molecule, bridging fundamental biochemistry with translational applications in inflammation and oncology.
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