| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5A1 |
| Uniprot No | P25705 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MLSVRVAAAVVRALPRRAGLVSRNALGSSFIAARNFHASNTHLQKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA |
| 分子量 | 86.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人线粒体ATP合成酶α亚基(ATP5F1A)的3篇代表性文献的简单概括：
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1. **文献名称**：*Structure and function of mitochondrial ATP synthase*
**作者**：Jonckheere, A. I., Smeitink, J. A., & Rodenburg, R. J.
**摘要**：系统综述了线粒体ATP合酶(包括α亚基ATP5F1A)的结构与功能，指出α亚基在催化ATP生成中的核心作用，并讨论了其突变与线粒体能量代谢疾病的关联。
2. **文献名称**：*A mitochondrial protein compendium elucidates complex I disease biology*
**作者**：Hejzlarova, K., et al.
**摘要**：通过蛋白质组学分析揭示了ATP5F1A在ATP合酶复合体组装中的关键地位，并发现其突变可导致ATP合成效率下降和神经退行性疾病表型。
3. **文献名称**：*ATP5F1A regulates mitochondrial cristae structure and cancer cell metabolism*
**作者**：Wang, Y., et al.
**摘要**：利用基因编辑技术证明ATP5F1A通过调控ATP合酶的寡聚化状态影响线粒体嵴形态，进而改变癌细胞代谢重编程和耐药性，提示其作为肿瘤治疗靶点的潜力。
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注：以上文献信息为模拟示例，实际引用需以具体论文内容为准。
The α-subunit of human mitochondrial ATP synthase (ATP5F1A, also known as ATP5A1) is a critical component of the F₁ domain within the FoF₁-ATP synthase complex. This enzyme, located in the mitochondrial inner membrane, catalyzes ATP production during oxidative phosphorylation by coupling proton translocation across the membrane to ATP synthesis. The α-subunit, encoded by the nuclear gene ATP5F1A, forms a heterohexameric catalytic core with three α- and three β-subunits. While the β-subunit primarily drives ATP hydrolysis/synthesis, the α-subunit stabilizes the complex and regulates conformational changes during catalysis.
ATP5F1A is highly conserved across eukaryotes, underscoring its essential role in cellular energy metabolism. Mutations in ATP5F1A are linked to mitochondrial disorders, such as Leigh syndrome, neurodegeneration, and cardiomyopathies, due to impaired ATP production. Recombinant ATP5F1A is typically expressed in bacterial or mammalian systems for structural and functional studies. Its 3D structure, resolved via crystallography and cryo-EM, reveals nucleotide-binding pockets and interfaces crucial for subunit interactions.
Research on recombinant ATP5F1A aids in understanding disease mechanisms, drug targeting for energy metabolism-related disorders, and evolutionary insights into ATP synthase's molecular architecture. Its study remains pivotal for advancing mitochondrial medicine and bioenergetics.
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