关键信息
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基因名
RAP1B
- 应用
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别名
RAP1B;Ras-related Protein Rap-1b
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P61224
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表达区间
1-181aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMREYKLVVLGSGGVGKSALTVQFVQGIFVE KYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAMRDLYMKNGQGFAL VYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQ GQNLARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSS C
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分子量
23 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RAP1B, a member of the Ras superfamily of small GTPases, plays a pivotal role in various cellular processes, including signal transduction, cell adhesion, and the regulation of cytoskeletal dynamics. Its aberrant expression and activation have been implicated in several pathophysiological conditions, particularly in cancer, where it may influence tumor growth and metastasis. The understanding of RAP1B's function has been significantly advanced through the study of its recombinant protein form, which allows researchers to investigate its biochemical properties and interactions in a controlled environment. By recombinantly expressing RAP1B, scientists can analyze its GTPase activity, identify downstream signaling pathways, and explore its role in cellular processes such as cell migration and proliferation. Furthermore, the production of RAP1B as a recombinant protein aids in the development of potential therapeutic strategies targeting aberrant RAP1B signaling. As the focus on small GTPases in cancer research continues to grow, the study of RAP1B as a recombinant protein provides valuable insights into its functional mechanisms and therapeutic potential, underscoring its significance as a target for cancer treatment and highlighting the importance of continuing research in this area.
