| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPZA2 |
| Uniprot No | P47755 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-286aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADLEEQ LSDEEKVRIA AKFIIHAPPG EFNEVFNDVR LLLNNDNLLR EGAAHAFAQY NLDQFTPVKI EGYEDQVLIT EHGDLGNGKF LDPKNRICFK FDHLRKEATD PRPCEVENAV ESWRTSVETA LRAYVKEHYP NGVCTVYGKK IDGQQTIIAC IESHQFQAKN FWNGRWRSEW KFTITPSTTQ VVGILKIQVH YYEDGNVQLV SHKDIQDSLT VSNEVQTAKE FIKIVEAAEN EYQTAISENY QTMSDTTFKA LRRQLPVTRT KIDWNKILSY KIGKEMQNA |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPZA2重组蛋白的3篇参考文献的简要总结(注：内容为模拟创作，非真实文献)：
1. **文献名称**：*Structural insights into CAPZA2-mediated actin capping mechanism*
**作者**：Li et al.
**摘要**：通过X射线晶体学解析CAPZA2重组蛋白的结构，揭示其与肌动蛋白丝结合的分子机制，阐明其通过C末端结构域调控肌动蛋白动态组装的生物学功能。
2. **文献名称**：*CAPZA2 knockdown impairs cell migration via dysregulated actin dynamics*
**作者**：Chen et al.
**摘要**：利用CAPZA2重组蛋白进行体外结合实验，证明其通过稳定肌动蛋白丝抑制乳腺癌细胞迁移，敲低CAPZA2导致细胞伪足形成异常并降低侵袭能力。
3. **文献名称**：*Recombinant CAPZA2 as a potential biomarker in neurodegenerative diseases*
**作者**：Park et al.
**摘要**：在大脑组织样本中发现CAPZA2表达异常，体外实验显示其重组蛋白可恢复神经元突触的肌动蛋白稳定性，提示其在阿尔茨海默病中的病理调控作用。
4. **文献名称**：*High-throughput screening of CAPZA2-interacting proteins using recombinant GST-tagged CAPZA2*
**作者**：Wang et al.
**摘要**：通过构建GST标记的CAPZA2重组蛋白，筛选出多个新型结合蛋白(如Cofilin和Arp2/3复合物)，揭示其在细胞骨架调控网络中的枢纽作用。
(以上内容基于领域知识模拟生成，实际文献需通过学术数据库验证。)
CAPZA2 (Capping Actin Protein of Muscle Z-Line Alpha Subunit 2) is a member of the CAPZ protein family, which regulates actin cytoskeleton dynamics by binding to the fast-growing (barbed) ends of actin filaments. This "capping" activity stabilizes actin networks, controls filament elongation, and influences critical cellular processes such as cell motility, morphogenesis, and intracellular trafficking. The CAPZ complex typically functions as a heterodimer, with CAPZA2 pairing with a beta subunit (CAPZB) to mediate its biological roles.
CAPZA2 is ubiquitously expressed but particularly abundant in tissues with high cytoskeletal remodeling demands, including muscle and neuronal cells. Studies link it to diverse physiological and pathological contexts. For instance, it modulates synaptic plasticity by regulating actin dynamics at neuronal synapses and maintains sarcomere integrity in muscle cells. Dysregulation of CAPZA2 has been implicated in cancer progression (e.g., influencing metastasis via altered cell migration), neurodegenerative disorders (e.g., impaired synaptic function), and cardiovascular diseases (e.g., compromised contractile machinery).
Recombinant CAPZA2 protein, produced through heterologous expression systems like *E. coli* or mammalian cells, retains the native structure and capping function. It serves as a key tool for *in vitro* studies, enabling researchers to dissect actin polymerization mechanisms, screen for cytoskeleton-targeting compounds, or map protein interaction networks. Its therapeutic potential is also being explored, particularly in diseases driven by aberrant actin dynamics. However, functional redundancy within the CAPZ family and context-dependent regulatory mechanisms remain active areas of investigation.
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