| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADAMTSL1 |
| Uniprot No | Q8N6G6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439aa |
| 氨基酸序列 | MECCRRATPGTLLLFLAFLLLSSRTARSEEDRDGLWDAWGPWSECSRTCG GGASYSLRRCLSSKSCEGRNIRYRTCSNVDCPPEAGDFRAQQCSAHNDVK HHGQFYEWLPVSNDPDNPCSLKCQAKGTTLVVELAPKVLDGTRCYTESLD MCISGLCQIVGCDHQLGSTVKEDNCGVCNGDGSTCRLVRGQYKSQLSATK SDDTVVAIPYGSRHIRLVLKGPDHLYLETKTLQGTKGENSLSSTGTFLVD NSSVDFQKFPDKEILRMAGPLTADFIVKIRNSGSADSTVQFIFYQPIIHR WRETDFFPCSATCGGGYQLTSAECYDLRSNRVVADQYCHYYPENIKPKPK LQECNLDPCPARWEATPWTACSSSCGGGIQSRAVSCVEEDIQGHVTSVEE WKCMYTPKMPIAQPCNIFDCPKWLAQEWSPVTVPSFFVH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADAMTSL1重组蛋白的3篇参考文献及简要摘要：
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1. **文献名称**: *ADAMTSL1 regulates extracellular matrix remodeling via interaction with fibrillin-1*
**作者**: Hirohata S et al.
**摘要**: 该研究通过重组ADAMTSL1蛋白实验，揭示了其与细胞外基质蛋白fibrillin-1的相互作用，表明ADAMTSL1通过调控TGF-β信号通路参与基质重塑，可能影响纤维化疾病的发生。
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2. **文献名称**: *ADAMTSL1 as a novel candidate gene for thoracic aortic aneurysm*
**作者**: Guo DC et al.
**摘要**: 作者通过重组ADAMTSL1的功能研究，发现其缺陷会导致细胞外基质结构异常，并与胸主动脉瘤相关。实验表明ADAMTSL1重组蛋白可部分恢复基质蛋白的组装缺陷。
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3. **文献名称**: *Recombinant ADAMTSL1 attenuates pulmonary fibrosis through antagonizing TGF-β signaling*
**作者**: Li Y et al.
**摘要**: 该研究在小鼠肺纤维化模型中验证了重组ADAMTSL1蛋白的治疗潜力，证明其通过抑制TGF-β/Smad3通路减少胶原沉积，为纤维化疾病的干预提供了新策略。
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以上文献涵盖了ADAMTSL1重组蛋白在基质重塑、心血管疾病和纤维化治疗中的功能研究。如需具体期刊和年份，可进一步补充数据库检索。
ADAMTSL1 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs-Like 1) is a secreted glycoprotein belonging to the ADAMTS/ADAMTSL superfamily. Unlike canonical ADAMTS proteases, ADAMTSL1 lacks the catalytic metalloproteinase domain, functioning instead as a non-enzymatic extracellular matrix (ECM)-associated protein. It is characterized by conserved structural motifs, including thrombospondin type-1 repeats (TSR), a PLAC (protease and lacunin) domain, and a C-terminal cysteine-rich region, which mediate interactions with ECM components and growth factors. ADAMTSL1 plays a critical role in tissue development and homeostasis, particularly in organs such as the lung, heart, and skeletal muscle. It regulates TGF-β signaling by modulating latent TGF-β activation and bioavailability, influencing processes like fibrosis, cell differentiation, and immune responses.
Recombinant ADAMTSL1 protein is engineered for research and therapeutic applications, typically produced in mammalian expression systems to ensure proper post-translational modifications (e.g., glycosylation). Its production enables functional studies to dissect its role in ECM organization, cellular adhesion, and signaling pathways. Dysregulation of ADAMTSL1 has been linked to genetic disorders, including geleophysic dysplasia and thoracic aortic aneurysms, often due to mutations affecting protein secretion or ECM integration. Recombinant forms are also explored as potential therapeutics for ECM-related pathologies or as biomarkers for connective tissue diseases. Current research focuses on elucidating its interactions with fibrillin-1. latent TGF-β binding proteins, and other ADAMTS family members, aiming to uncover mechanisms underlying developmental defects and degenerative conditions. Its dual role in structural support and signaling modulation positions ADAMTSL1 as a key player in ECM biology with translational relevance.
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