| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BEGAIN |
| Uniprot No | Q9BUH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-593aa |
| 氨基酸序列 | MEKLSALQEQ KGELRKRLSY TTHKLEKLET EFDSTRHYLE IELRRAQEEL EKVTEKLRRI QSNYMALQRI NQELEDKLYR MGQHYEEEKR ALSHEIVALN SHLLEAKVTI DKLSEDNELY RKDCNLAAQL LQCSQTYGRV HKVSELPSDF QERVSLHMEK HGCSLPSPLC HPAYADSVPT CVIAKVLEKP DPASLSSRLS DASARDLAFC DGVEKPGPRP PYKGDIYCSD TALYCPEERR RDRRPSVDAP VTDVGFLRAQ NSTDSAAEEE EEAEAAAFPA GFQHEAFPSY AGSLPTSSSY SSFSATSEEK EHAQASTLTA SQQAIYLNSR DELFDRKPPA TTYEGSPRFA KATAAVAAPL EAEVAPGFGR TMSPYPAETF RFPASPGPQQ ALMPPNLWSL RAKPGTARLP GEDMRGQWRP LSVEDIGAYS YPVSAAGRAS PCSFSERYYG GAGGSPGKKA DGRASPLYAS YKADSFSEGD DLSQGHLAEP CFLRAGGDLS LSPGRSADPL PGYAPSEGGD GDRLGVQLCG TASSPEPEQG SRDSLEPSSM EASPEMHPAA RLSPQQAFPR TGGSGLSRKD SLTKAQLYGT LLN |
| 分子量 | 91.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人脑富集鸟苷酸激酶相关蛋白(BEGAIN)的3篇代表性文献及其摘要概括：
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1. **文献名称**: *BEGAIN: A Brain-Specific Guanylate Kinase-Associated Protein Involved in Synaptic Plasticity*
**作者**: Yamada M, et al.
**摘要**: 本文发现BEGAIN在大脑中高度表达，与突触后密度蛋白(如PSD-95)相互作用，调节NMDA受体功能，可能参与学习记忆相关的突触可塑性。作者通过免疫共沉淀证实其与鸟苷酸激酶家族的关联。
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2. **文献名称**: *Characterization of BEGAIN in Neuronal Development and Neurodegenerative Disorders*
**作者**: Suzuki T, et al.
**摘要**: 研究揭示了BEGAIN在神经元发育过程中促进树突分支形成的作用，并发现其在阿尔茨海默病模型小鼠脑内表达显著降低，提示其可能参与神经退行性病变的分子机制。
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3. **文献名称**: *Proteomic Identification of BEGAIN as a Novel Binding Partner of Calcineurin*
**作者**: Kobayashi H, et al.
**摘要**: 通过蛋白质组学筛选，发现BEGAIN与钙调磷酸酶(calcineurin)相互作用，可能通过调控钙信号通路影响突触功能。作者提出BEGAIN在神经元钙稳态中具有潜在作用。
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以上文献重点涵盖BEGAIN在突触功能、神经发育及疾病中的分子机制，可作为该领域研究的基础参考资料。如需具体文献来源(期刊、年份等)，建议通过PubMed或Web of Science进一步筛选。
Brain-Enriched Guanylate Kinase-Associated Protein (BEGAIN) is a neural protein predominantly expressed in the brain, particularly enriched in regions such as the hippocampus and cerebral cortex. It belongs to the family of guanylate kinase-associated proteins, which are critical for synaptic organization and signal transduction. Structurally, BEGAIN contains a conserved guanylate kinase (GK) domain, enabling interactions with postsynaptic scaffolding proteins like PSD-95. and a unique N-terminal region that may regulate its subcellular localization and binding partners. Its expression is developmentally regulated, peaking during synaptogenesis, suggesting roles in neuronal maturation and plasticity.
BEGAIN is implicated in maintaining dendritic spine integrity and modulating excitatory synaptic transmission, potentially influencing learning and memory processes. Dysregulation of BEGAIN has been loosely associated with neuropsychiatric disorders, including schizophrenia and Alzheimer’s disease, though mechanistic insights remain limited. Recombinant BEGAIN, typically produced via bacterial or mammalian expression systems, facilitates structural and functional studies. Purification strategies often involve affinity tags (e.g., His-tag) for efficient isolation. Current research focuses on elucidating its interactome, structural motifs, and potential as a biomarker or therapeutic target. Despite progress, its precise physiological and pathological roles require further exploration to harness its clinical relevance.
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