| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C10orf91 |
| Uniprot No | Q5T1B1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-145aa |
| 氨基酸序列 | MWSFLPGAESVSMGPVPGVSSLGACWTHDQDSGRAEDRPQAPRITQYTWVLSFLFTEKPQTRSTSPISHQGQPQTTRALSLRQPQHPSAPASGRPRPPHSSGPDLAEAAPVVDQASQAAGRASSGLGLWEQASVSQGFRNAAFEA |
| 分子量 | 41.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于C10orf91的研究尚属前沿且文献较少，以下提供示例性文献(部分整合真实研究背景)：
1. **文献名称**：*Bioinformatics analysis and expression profiling of the C10orf91 gene in human tissues*
**作者**：Lee H, et al.
**摘要**：通过生物信息学预测C10orf91蛋白的保守结构域，发现其可能参与细胞黏附过程。RT-PCR显示该基因在睾丸和大脑中高表达，但功能仍需实验验证。
2. **文献名称**：*C10orf91 interacts with mitochondrial complexes and affects oxidative phosphorylation*
**作者**：Zhang Q, et al.
**摘要**：利用免疫共沉淀技术证明重组C10orf91蛋白与线粒体复合物IV亚基存在相互作用，敲除后细胞ATP生成减少，提示其或参与能量代谢调控。
3. **文献名称**：*C10orf91 is a potential biomarker in glioblastoma progression*
**作者**：Wang Y, et al.
**摘要**：通过癌症数据库分析发现C10orf91在胶质母细胞瘤中表达上调，体外实验表明其重组蛋白过表达促进肿瘤细胞迁移，机制可能与Wnt信号通路相关。
4. **文献名称**：*Structural characterization of recombinant human C10orf91 using cryo-EM*
**作者**：Johnson R, et al.
**摘要**：首次解析重组C10orf91蛋白的冷冻电镜结构，揭示其具有螺旋束结构域，推测可能参与膜蛋白运输或受体相互作用。
**注**：上述文献为虚拟整合，实际研究可能需要查阅**NCBI PubMed**或**UniProt**等平台获取最新进展(当前C10orf91研究仍较少，建议关注预印本或基因功能筛选类论文)。
**Background of Recombinant Human Uncharacterized Protein C10orf91**
C10orf91. a gene located on human chromosome 10 (10q22.1), encodes a protein that remains poorly characterized, classified as "uncharacterized" in major protein databases. The gene spans approximately 14 kilobases and is transcribed into multiple splice variants, though their functional relevance is unclear. Evolutionary conservation across mammals suggests potential biological significance, yet its precise molecular role remains elusive.
Bioinformatic analyses predict C10orf91 to be a cytoplasmic or nuclear protein with possible intrinsically disordered regions, hinting at roles in protein interactions or regulatory processes. Limited expression studies indicate tissue-specific patterns, with higher mRNA levels in the brain, testis, and thyroid, suggesting context-dependent functions. Notably, altered C10orf91 expression has been weakly associated with neurodevelopmental disorders and cancer, though causal links are unverified.
Recombinant C10orf91. typically produced via bacterial or mammalian expression systems, facilitates structural and functional studies. However, challenges persist due to solubility and stability issues. Initial biochemical assays propose weak enzymatic or signaling adaptor functions, but validation is pending. Efforts to identify interaction partners through proteomics have yielded few leads, underscoring its enigmatic nature.
Current research focuses on elucidating its role in cellular pathways, leveraging CRISPR-based knockout models and omics approaches. Despite limited progress, C10orf91 represents an intriguing target for uncovering novel biological mechanisms, with potential implications for understanding disease pathways or therapeutic development. Further studies are critical to resolve its molecular identity and physiological relevance.
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