关键信息
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基因名
NEUROD4
- 应用
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别名
NEUROD4; ATH3; ATOH3; BHLHA4; Neurogenic differentiation factor 4; NeuroD4; Class A basic helix-loop-helix protein 4; bHLHa4; Protein atonal homolog 3; ATH-3; Atoh3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9HD90
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表达区间
1-331 aa
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氨基酸序列
MSKTFVKSKE MGELVNTPSW MDKGLGSQNE VKEEESRPGT YGMLSSLTEE HDSIEEEEEE EEDGEKPKRR GPKKKKMTKA RLERFRARRV KANARERTRM HGLNDALDNL RRVMPCYSKT QKLSKIETLR LARNYIWALS EVLETGQTPE GKGFVEMLCK GLSQPTSNLV AGCLQLGPQS VLLEKHEDKS PICDSAISVH NFNYQSPGLP SPPYGHMETH LLHLKPQVFK SLGESSFGSH LPDCSTPPYE GPLTPPLSIS GNFSLKQDGS PDLEKSYSFM PHYPSSSLSS GHVHSTPFQA GTPRYDVPID MSYDSYPHHG IGTQLNTVFT E
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分子量
37.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
NEUROD4, a member of the NEUROD family of basic helix-loop-helix transcription factors, plays a crucial role in the development and differentiation of neural lineages, particularly in the formation of neurons and insulin-producing beta cells in the pancreas. Its expression is tightly regulated during embryonic development, and it is implicated in neurogenesis, tumorgenesis, and cellular reprogramming. The study of NEUROD4 has gained prominence due to its potential therapeutic applications, particularly in regenerative medicine and diabetes treatment. Understanding the mechanisms by which NEUROD4 influences cell fate and its interactions with other signaling pathways can provide insights for developing novel strategies for neurodegenerative diseases and metabolic disorders. Researchers have increasingly focused on the production and characterization of recombinant NEUROD4 proteins to elucidate its structure-function relationships and to explore its capabilities in promoting neuronal differentiation in various stem cell models. This recombinant approach enables detailed investigations into NEUROD4’s functional domains and its role in transcriptional regulation, thereby paving the way for future biotechnological applications in cell therapy and biotechnology. The ongoing research aims to harness the potential of NEUROD4 in therapeutic contexts, emphasizing its significance in both fundamental biology and clinical applications.
