关键信息
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基因名
NFKBIE
- 应用
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别名
I kappa B epsilon; I-kappa-B-epsilon; IkappaBepsilon; IkB E; IkB-E; IkB-epsilon; IKBE; IKBE_HUMAN; MGC72568; NF kappa B inhibitor epsilon; NF kappa BIE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; NFkappa BIE; NFkappaB inhibitor epsilon; NFKBIE; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon; OTTHUMP00000016522; Slc35b2; solute carrier family 35; member B2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00221
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表达区间
1-500 aa
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氨基酸序列
MNQRRSESRP GNHRLQAYAE PGKGDSGGAG PLSGSARRGR GGGGAIRVRR PCWSGGAGRG GGPAWAVRLP TVTAGWTWPA LRTLSSLRAG PSEPHSPGRR PPRAGRPLCQ ADPQPGKAAR RSLEPDPAQT GPRPARAAGM SEARKGPDEA EESQYDSGIE SLRSLRSLPE STSAPASGPS DGSPQPCTHP PGPVKEPQEK EDADGERADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH VGALSPQQLE ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR PEPGRGTSHS LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE GTSGKTALHL AVETQERGLV QFLLQAGAQV DARMLNGCTP LHLAAGRGLM GISSTLCKAG ADSLLRNVED ETPQDLTEES LVLLPFDDLK ISGKLLLCTD
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分子量
52.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
NFKBIE, also known as IκBε (Inhibitor of Nuclear Factor kappa-B epsilon), is a member of the IκB family of proteins, which are crucial regulators of the NF-κB (Nuclear Factor kappa-light-chain-enhancer of activated B cells) signaling pathway. This pathway plays a vital role in controlling immune responses, inflammation, cell survival, and proliferation. Upon stimulation by various cytokines or stress signals, NF-κB dimers, typically composed of p65 (RelA) and p50, are released from their inhibitor complexes, allowing them to translocate to the nucleus and initiate the transcription of target genes involved in immune and inflammatory responses. NFKBIE specifically functions to inhibit the NF-κB pathway by sequestering NF-κB dimers in the cytoplasm and preventing their nuclear translocation. Elevated levels of NFKBIE have been associated with certain cancers and autoimmune diseases, indicating its potential as a therapeutic target. Understanding the functional dynamics of NFKBIE, including its expression regulation and interaction with other NF-κB components, is essential for developing novel therapeutic strategies aimed at modulating NF-κB activity. The production and study of recombinant NFKBIE protein can provide valuable insights into its structural properties, mechanism of action, and role in disease pathology, ultimately contributing to the development of targeted interventions in NF-κB-related disorders. Research exploring the biochemical and biophysical characteristics of this protein may pave the way for innovative approaches in both basic and clinical research focused on immune modulation and inflammation control.
