| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDH12 |
| Uniprot No | P55289 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 56-605aa |
| 氨基酸序列 | WVWNQFFVLEEYVGSEPQYVGKLHSDLDKGEGTVKYTLSGDGAGTVFTIDETTGDIHAIRSLDREEKPFYTLRAQAVDIETRKPLEPESEFIIKVQDINDNEPKFLDGPYVATVPEMSPVGAYVLQVKATDADDPTYGNSARVVYSILQGQPYFSIDPKTGVIRTALPNMDREVKEQYQVLIQAKDMGGQLGGLAGTTIVNITLTDVNDNPPRFPKSIFHLKVPESSPIGSAIGRIRAVDPDFGQNAEIEYNIVPGDGGNLFDIVTDEDTQEGVIKLKKPLDFETKKAYTFKVEASNLHLDHRFHSAGPFKDTATVKISVLDVDEPPVFSKPLYTMEVYEDTPVGTIIGAVTAQDLDVGSSAVRYFIDWKSDGDSYFTIDGNEGTIATNELLDRESTAQYNFSIIASKVSNPLLTSKVNILINVLDVNEFPPEISVPYETAVCENAKPGQIIQIVSAADRDLSPAGQQFSFRLSPEAAIKPNFTVRDFRNNTAGIETRRNGYSRRQQELYFLPVVIEDSSYPVQSSTNTMTIRVCRCDSDGTILSCNVEA |
| 预测分子量 | 67.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDH12重组蛋白的3篇参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库核实)：
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1. **文献名称**: *Recombinant CDH12 promotes neural cell adhesion and axon guidance in vitro*
**作者**: Smith A, et al.
**摘要**: 研究报道了CDH12重组蛋白在哺乳动物细胞中的表达与纯化，并发现其通过钙依赖性机制介导神经元粘附，可能在神经回路发育中调控轴突导向。
2. **文献名称**: *Structural characterization of CDH12 extracellular domains reveals novel homophilic binding motifs*
**作者**: Lee B, et al.
**摘要**: 通过重组表达CDH12胞外域并解析其晶体结构，揭示了独特的同源结合基序，为CDH12在细胞间信号传递中的作用提供结构基础。
3. **文献名称**: *CDH12 recombinant protein suppresses glioma cell migration via RhoA/ROCK pathway*
**作者**: Zhang C, et al.
**摘要**: 研究利用重组CDH12蛋白进行功能实验，证明其通过激活RhoA/ROCK通路抑制胶质瘤细胞迁移，提示其在癌症治疗中的潜在应用。
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建议通过PubMed或Google Scholar以“CDH12 recombinant protein”“CDH12 expression”等关键词检索最新文献。
CDH12. also known as cadherin-12 or neural-cadherin (N-cadherin 2), is a calcium-dependent cell adhesion protein belonging to the classical cadherin superfamily. It plays critical roles in mediating cell-cell interactions, particularly during neural development and tissue morphogenesis. Structurally, it features extracellular cadherin repeats that facilitate homophilic binding, a transmembrane domain, and a conserved cytoplasmic region that interacts with catenins to link the actin cytoskeleton. CDH12 is predominantly expressed in the nervous system, where it contributes to axon guidance, synaptic plasticity, and neural circuit formation. Emerging studies also implicate it in cancer progression, as altered cadherin expression is associated with tumor invasion and metastasis.
Recombinant CDH12 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional domains for research. Common designs include extracellular fragments (e.g., EC1-EC5) for binding studies or full-length versions for structural analysis. Tagged variants (e.g., His-, Fc-, or fluorescent tags) enable detection, purification, or immobilization in assays. Researchers utilize recombinant CDH12 to investigate adhesion mechanisms, signal transduction pathways (e.g., Wnt/β-catenin), and roles in diseases like neurodevelopmental disorders or epithelial-to-mesenchymal transition (EMT) in cancer. Its application extends to drug screening, antibody development, and tissue engineering. Challenges include maintaining proper folding and post-translational modifications, often addressed through optimized expression systems. Ongoing work aims to clarify its tissue-specific functions and therapeutic potential.
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