| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGM3 |
| Uniprot No | Q08188 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-467aa |
| 氨基酸序列 | AALGVQSIN WQTAFNRQAH HTDKFSSQEL ILRRGQNFQV LMIMNKGLGS NERLEFIVST GPYPSESAMT KAVFPLSNGS SGGWSAVLQA SNGNTLTISI SSPASAPIGR YTMALQIFSQ GGISSVKLGT FILLFNPWLN VDSVFMGNHA EREEYVQEDA GIIFVGSTNR IGMIGWNFGQ FEEDILSICL SILDRSLNFR RDAATDVASR NDPKYVGRVL SAMINSNDDN GVLAGNWSGT YTGGRDPRSW NGSVEILKNW KKSGFSPVRY GQCWVFAGTL NTALRSLGIP SRVITNFNSA HDTDRNLSVD VYYDPMGNPL DKGSDSVWNF HVWNEGWFVR SDLGPSYGGW QVLDATPQER SQGVFQCGPA SVIGVREGDV QLNFDMPFIF AEVNADRITW LYDNTTGKQW KNSVNSHTIG RYISTKAVGS NARMDVTDKY KYPEGSDQER QVFQKALGKL KPNTPFA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGM3重组蛋白的模拟参考文献示例(非真实文献，供参考)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human Transglutaminase 3 (TGM3) in Epithelial Differentiation*
**作者**：Smith A, et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了重组TGM3蛋白，并验证其在体外角质形成细胞分化中的关键作用，发现TGM3通过交联表皮结构蛋白参与皮肤屏障形成。
2. **文献名称**：*TGM3 Recombinant Protein Suppresses Tumor Invasion in Esophageal Cancer Models*
**作者**：Li X, et al.
**摘要**：文章利用大肠杆菌表达系统获得高纯度TGM3重组蛋白，实验表明其通过抑制EGFR信号通路显著降低食管癌细胞迁移和侵袭能力，提示其潜在抑癌作用。
3. **文献名称**：*Structural Analysis of TGM3 Catalytic Domain via Recombinant Protein Crystallography*
**作者**：Gupta R, et al.
**摘要**：通过昆虫细胞表达系统制备TGM3催化结构域重组蛋白并解析其晶体结构，揭示了钙离子结合位点及底物识别机制，为开发靶向抑制剂提供依据。
4. **文献名称**：*Recombinant TGM3 Accelerates Wound Healing in Diabetic Mouse Skin*
**作者**：Chen Y, et al.
**摘要**：研究证明局部应用重组TGM3蛋白可通过增强表皮层粘连蛋白交联，改善糖尿病模型小鼠的伤口愈合速度与表皮完整性，具有临床应用潜力。
**Background of TGM3 Recombinant Protein**
Transglutaminase 3 (TGM3), a member of the transglutaminase enzyme family, plays a critical role in epidermal homeostasis and keratinocyte differentiation. Primarily expressed in stratified squamous epithelia, including skin and hair follicles, TGM3 catalyzes the cross-linking of structural proteins like involucrin and loricrin through ε-(γ-glutamyl)lysine bonds, contributing to the formation of the cornified envelope—a key component of the skin barrier. This enzymatic activity is calcium-dependent and regulates tissue integrity, wound healing, and protection against environmental stressors.
Structurally, TGM3 contains an N-terminal β-sandwich domain, a catalytic core, and two C-terminal β-barrels. Its expression is tightly regulated during epithelial differentiation, mediated by transcription factors such as p63 and Notch signaling. Dysregulation of TGM3 has been implicated in dermatological disorders (e.g., psoriasis, atopic dermatitis) and cancers, where abnormal cross-linking may promote tumor progression or resistance to apoptosis.
Recombinant TGM3 protein, produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), retains enzymatic activity and structural fidelity, enabling *in vitro* studies of its biochemical properties and interactions. It serves as a vital tool for investigating epidermal biology, screening therapeutic agents targeting skin diseases, and exploring TGM3's role in cancer metastasis. Additionally, recombinant TGM3 aids in developing diagnostic assays for autoimmune conditions linked to anti-TGM3 antibodies.
Overall, TGM3 recombinant protein bridges basic research and clinical applications, offering insights into epithelial pathophysiology and potential therapeutic strategies.
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