关键信息
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基因名
PPP1R3C
- 应用
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别名
PPP1R3C；Laforin
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UQK1
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表达区间
1-317aa
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氨基酸序列
MSCTRMIQVL DPRPLTSSVM PVDVAMRLCL AHSPPVKSFL GPYDEFQRRH FVNKLKPLKS CLNIKHKAKS QNDWKCSHNQ AKKRVVFADS KGLSLTAIHV FSDLPEEPAW DLQFDLLDLN DISSALKHHE EKNLILDFPQ PSTDYLSFRS HFQKNFVCLE NCSLQERTVT GTVKVKNVSF EKKVQIRITF DSWKNYTDVD CVYMKNVYGG TDSDTFSFAI DLPPVIPTEQ KIEFCISYHA NGQVFWDNND GQNYRIVHVQ WKPDGVQTQM APQDCAFHQT SPKTELESTI FGSPRLASGL FPEWQSWGRM ENLASYR
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分子量
36.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PPP1R3C, also known as Protein Phosphatase 1 Regulatory Subunit 3C, is a member of the PPP1R3 family that plays a critical role in regulating protein phosphatase 1 (PP1) activity, which is essential for various cellular processes, including glycogen metabolism, cell signaling, and apoptosis. The importance of PPP1R3C in these pathways has garnered significant research interest, particularly in understanding its role in metabolic disorders such as diabetes and obesity. Studies have indicated that PPP1R3C may influence insulin signaling and glucose homeostasis, making it a potential target for therapeutic interventions. Furthermore, the characterization of PPP1R3C as a regulatory subunit highlights its capability to modulate PP1's substrate specificity and activity, which is vital for fine-tuning cellular responses. Recent advances in recombinant protein technology have enabled the production of purified PPP1R3C proteins, facilitating detailed structural and functional analyses. These studies aim to elucidate the molecular mechanisms through which PPP1R3C affects PP1 activity and its broader implications in cellular metabolism and disease. Understanding the functional dynamics of this protein could provide insights into novel strategies for treating metabolic-related diseases, and thus, its recombinant study is of paramount significance in both basic and applied biomedical research.
