Cat: PA1000-3018

Recombinant Human SRP14 Protein,His

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关键信息

  • 基因名

    SRP14

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    SRP14;Signal recognition particle 14 kDa Protein

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P37108

  • 表达区间

    1-160aa

  • 氨基酸序列

    MGSSHHHHHHSSGLVPRGSHMGSHMVLLESEQFLTELTRLFQKCRTSGSV YITLKKYDGRTKPIPKKGTVEGFEPADNKCLLRATDGKKK ISTVVSSKEVNKFQMAYSNLLRANMDGLKKRDKKNKTKKTKAAAAAAAAA PAAAATAATTAATTAATAAQ

  • 分子量

    17 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

SRP14, or Signal Recognition Particle 14 kDa protein, plays a critical role in the signal recognition particle (SRP) complex, which is essential for targeting nascent polypeptides to the endoplasmic reticulum (ER) for proper translocation and processing. The SRP pathway is fundamental for maintaining cellular homeostasis and ensuring that protein synthesis occurs in the correct cellular compartment. Research into SRP14 is particularly important due to its involvement in various physiological processes and its potential implications in diseases linked to protein misfolding and trafficking. Understanding the structure and function of SRP14 can provide insights into the molecular mechanisms of SRP-dependent protein targeting, highlighting its importance in both basic biology and potential therapeutic applications. Furthermore, as a recombinant protein, SRP14 can be utilized in biochemical assays to elucidate its interactions and regulatory mechanisms within the SRP complex. This research holds promise for uncovering novel strategies to modulate protein synthesis pathways, which may have significant implications for developing treatments for diseases such as cystic fibrosis, cancer, and neurodegenerative disorders, where protein misfolding is a key factor. Thus, the study of SRP14 not only enriches our understanding of cellular processes but also paves the way for innovative approaches to therapeutic intervention in a variety of diseases.

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