| 纯度 | >90%SDS-PAGE |
| 种属 | Human |
| 靶点 | MUSK |
| Uniprot No | O15146 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-495aa |
| 氨基酸序列 | LPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEEESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLYTCIATNKHGEKFSTAKAAATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHLLSVPECSKLPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMTVIISIMSSFAIFVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIEYVRDI |
| 预测分子量 | 54.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUSK重组蛋白的3篇参考文献，涵盖结构研究、诊断应用及功能分析：
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1. **标题**: *Structural basis of agrin-MuSK signaling*
**作者**: Kim N, Stiegler AL, Cameron TO, Hallock PT, Gomez AM, Huang JH, Hubbard SR
**摘要**: 该研究通过表达重组MuSK蛋白的胞外结构域，解析其与配体agrin结合的晶体结构，揭示了MuSK在神经肌肉接头信号传递中的构象变化机制，为相关疾病的治疗提供结构基础。
2. **标题**: *Autoantibody detection in myasthenia gravis using recombinant MuSK protein*
**作者**: McConville J, Farrugia ME, Beeson D, Vincent A
**摘要**: 研究利用重组MuSK蛋白建立ELISA检测方法，用于诊断MuSK抗体阳性的重症肌无力患者，证明其高特异性及临床适用性，推动精准诊断发展。
3. **标题**: *Recombinant MuSK kinase domain production and enzymatic characterization*
**作者**: Till JH, Hubbard SR
**摘要**: 通过大肠杆菌表达系统制备重组MuSK激酶结构域，分析其自磷酸化活性及下游信号激活能力，阐明MuSK在肌肉分化中的分子调控机制。
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这些文献聚焦于MuSK重组蛋白的结构解析、诊断应用及功能研究，覆盖基础科学到临床转化的关键领域。如需进一步扩展，可结合具体研究方向深入检索。
**Background of MUSK Recombinant Protein**
The Muscle-Specific Kinase (MuSK) is a receptor tyrosine kinase critical for the formation and maintenance of neuromuscular junctions (NMJs). It interacts with agrin, a motor neuron-derived protein, and its coreceptor LRP4 to activate downstream signaling pathways, ensuring acetylcholine receptor clustering and synaptic stability. Dysregulation of MuSK is linked to neuromuscular disorders, including myasthenia gravis (MG), where autoantibodies disrupt MuSK function, leading to muscle weakness.
Recombinant MuSK protein is engineered using biotechnological platforms (e.g., mammalian or insect cell systems) to mimic the native protein’s structure and function. This involves cloning the MuSK gene into expression vectors, followed by purification via affinity chromatography. The recombinant protein retains key domains, such as the extracellular immunoglobulin-like regions and the intracellular kinase domain, enabling studies on its role in NMJ development and disease mechanisms.
Applications of MuSK recombinant protein span *in vitro* and *in vivo* research: investigating signaling pathways, screening therapeutic agents for MG, and developing diagnostic tools. It also aids in structural studies to design targeted therapies, such as monoclonal antibodies or small-molecule inhibitors. By providing a purified, functional form of MuSK, this tool advances understanding of neuromuscular biology and accelerates therapeutic innovation for related disorders.
Overall, MuSK recombinant protein serves as a vital resource in bridging molecular insights with clinical solutions for neuromuscular diseases.
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