纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CXorf34 |
Uniprot No | Q96GJ1 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-504aa |
氨基酸序列 | MAGLKRRVPLHSLRYFISMVGLFSKPGLLPWYARNPPGWSQLFLGTVCKGDFTRVIATKCQKGQKSQKKPSHLGPLDGSWQERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVSVTTAVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYLGTWRDGNVVCVQSNHLKNIPEKHSQVAQYYEVFLRQSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEELHVQKEIVKEFFIRGPGAACGLTSLYFQESTMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGELTGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSKEDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR |
分子量 | 82.9 kDa |
蛋白标签 | GST-tag at N-terminal |
缓冲液 | 0 |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CXorf34蛋白的参考文献示例(注:由于该蛋白研究较少,以下内容为模拟虚构,供参考):
1. **标题**:*Molecular characterization and functional analysis of recombinant human CXorf34 protein*
**作者**:Smith A, et al.
**摘要**:研究通过大肠杆菌系统成功表达并纯化重组人CXorf34蛋白,发现其在体外模型中可能参与DNA损伤修复过程,并通过质谱分析验证其与其他修复蛋白的相互作用。
2. **标题**:*CXorf34 overexpression induces apoptosis in human cancer cell lines*
**作者**:Wang Y, et al.
**摘要**:报道重组CXorf34蛋白在多种肿瘤细胞系中的促凋亡活性,揭示了其可能通过激活线粒体途径调控caspase-3信号通路,为癌症靶向治疗提供潜在靶点。
3. **标题**:*Structural insights into the CXorf34 protein: Implications for X-linked genetic disorders*
**作者**:Johnson R, et al.
**摘要**:解析了重组CXorf34蛋白的晶体结构,提出其N端结构域可能与X染色体相关遗传疾病的致病突变相关,并探讨了功能缺失对神经发育的影响。
4. **标题**:*Proteomic profiling identifies CXorf34 as a novel interactor of the BRCA1 complex*
**作者**:Chen L, et al.
**摘要**:利用重组CXorf34蛋白进行免疫共沉淀实验,发现其与BRCA1复合物成员存在相互作用,提示其在乳腺癌发生中的潜在调控作用。
**注意**:以上文献均为示例,具体研究需查询真实数据库(如PubMed)。若需实际文献,建议使用关键词“CXorf34 recombinant protein”或“CXorf34 function”进一步检索。
Recombinant human CXorf34 protein is a genetically engineered form of the protein encoded by the *CXorf34* gene (Chromosome X Open Reading Frame 34), located on the X chromosome (Xq26.3). Though its exact biological function remains unclear, CXorf34 is speculated to play roles in cellular processes such as transcriptional regulation or signaling, supported by conserved structural domains observed in homologs. Interest in this protein has grown due to its potential links to human diseases. For instance, aberrant expression of CXorf34 has been reported in certain cancers, including prostate and breast cancers, suggesting it may influence tumor progression or therapy resistance. Additionally, its X-chromosome localization hints at possible roles in sex-linked disorders or developmental conditions.
The recombinant form is typically produced in bacterial or mammalian expression systems, enabling controlled study of its biochemical properties, interactions, and functional pathways. Researchers utilize recombinant CXorf34 as a tool to generate antibodies, map binding partners, or explore its involvement in disease mechanisms. Its recombinant nature ensures high purity and reproducibility, critical for experimental consistency. Despite limited functional data, ongoing studies aim to clarify its role in cellular homeostasis and pathology, potentially uncovering therapeutic targets or diagnostic biomarkers. This protein represents a focal point in bridging genomic data with functional protein analysis in the post-genomic era. (Word count: 199)
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