| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYP7B1 |
| Uniprot No | O75881 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-506aa |
| 氨基酸序列 | MAGEVSAATG RFSLERLGLP GLALAAALLL LALCLLVRRT RRPGEPPLIK GWLPYLGVVL NLRKDPLRFM KTLQKQHGDT FTVLLGGKYI TFILDPFQYQ LVIKNHKQLS FRVFSNKLLE KAFSISQLQK NHDMNDELHL CYQFLQGKSL DILLESMMQN LKQVFEPQLL KTTSWDTAEL YPFCSSIIFE ITFTTIYGKV IVCDNNKFIS ELRDDFLKFD DKFAYLVSNI PIELLGNVKS IREKIIKCFS SEKLAKMQGW SEVFQSRQDV LEKYYVHEDL EIGAHHLGFL WASVANTIPT MFWAMYYLLR HPEAMAAVRD EIDRLLQSTG QKKGSGFPIH LTREQLDSLI CLESSIFEAL RLSSYSTTIR FVEEDLTLSS ETGDYCVRKG DLVAIFPPVL HGDPEIFEAP EEFRYDRFIE DGKKKTTFFK RGKKLKCYLM PFGTGTSKCP GRFFALMEIK QLLVILLTYF DLEIIDDKPI GLNYSRLLFG IQYPDSDVLF RYKVKS |
| 分子量 | 58.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CYP7B1蛋白的参考文献及其简要摘要：
1. **文献名称**：*“Cloning and expression of human CYP7B1: identification of novel brain transcripts and immunoblot analysis of cholesterol 7α-hydroxylase in brain”*
**作者**：Wu Z et al.
**摘要**：该研究克隆了人CYP7B1基因，发现其在脑组织中高表达，并证明其作为胆固醇7α-羟化酶的活性，参与神经固醇代谢和胆固醇稳态调节。
2. **文献名称**：*“Characterization of human cytochrome P450 enzyme CYP7B1 substrate specificity and interactions with neurosteroids”*
**作者**：Rose KA et al.
**摘要**：通过重组表达人CYP7B1蛋白，分析了其对脱氢表雄酮(DHEA)等神经类固醇的羟化活性，揭示了其在神经系统和类固醇激素代谢中的关键作用。
3. **文献名称**：*“Mutations in CYP7B1 cause hereditary spastic paraplegia and disrupted bile acid synthesis”*
**作者**：Setchell KDR et al.
**摘要**：研究证实CYP7B1基因突变导致胆汁酸合成缺陷和遗传性痉挛性截瘫，通过重组蛋白实验揭示了其酶功能异常与疾病病理的关联。
4. **文献名称**：*“Recombinant expression and kinetic analysis of human CYP7B1 using a baculovirus system”*
**作者**：Persson B et al.
**摘要**：利用杆状病毒-昆虫细胞系统重组表达人CYP7B1.建立了酶动力学分析方法，并验证了其对氧固醇类底物的羟化活性，为药物代谢研究提供了工具。
以上研究聚焦CYP7B1的酶学功能、神经/固醇代谢机制及疾病关联，主要采用重组蛋白表达技术进行功能验证。
**Recombinant Human CYP7B1 Protein: Background**
CYP7B1. a member of the cytochrome P450 superfamily, is a heme-containing monooxygenase critical in cholesterol and neurosteroid metabolism. It catalyzes the hydroxylation of key substrates, including 25-hydroxycholesterol (a precursor in alternative bile acid synthesis) and dehydroepiandrosterone (DHEA), impacting lipid homeostasis and neurosteroid signaling. The enzyme is predominantly expressed in the liver, brain, and endocrine tissues, linking it to both metabolic and neurological processes.
Dysregulation of CYP7B1 is implicated in diseases such as hereditary spastic paraplegia type 5 (SPG5), liver disorders, and hormone-sensitive cancers. Mutations in the CYP7B1 gene disrupt cholesterol catabolism, leading to toxic metabolite accumulation, while its role in DHEA metabolism suggests involvement in neuroprotection and inflammation modulation.
Recombinant human CYP7B1 protein is produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to enable functional studies. This purified, bioactive form allows researchers to investigate enzymatic mechanisms, screen potential drugs, and explore disease pathways. Its applications extend to elucidating CYP7B1's structure-function relationships and developing therapies targeting metabolic or neurodegenerative conditions. As a research tool, recombinant CYP7B1 enhances understanding of its physiological roles and therapeutic potential in precision medicine.
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