| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DENND1B |
| Uniprot No | Q6P3S1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396aa |
| 氨基酸序列 | MAAAPREEKRWPQPVFSNPVVLWKFPEDFGDQEILQSVPKFCFPFDVERVSQNQVGQHFTFVLTDIESKQRFGFCRLTSGGTICLCILSYLPWFEVYYKLLNTLADYLAKELENDLNETLRSLYNHPVPKANTPVNLSVHSYFIAPDVTGLPTIPESRNLTEYFVAVDVNNMLQLYASMLHERRIVIISSKLSTLTACIHGSAALLYPMYWQHIYIPVLPPHLLDYCCAPMPYLIGIHSSLIERVKNKSLEDVVMLNVDTNTLESPFSDLNNLPSDVVSALKNKLKKQSTATGDGVARAFLRAQAALFGYYRDALRYKPGEPITFCEESFVKHRSSVMKQFLETAINLQLFKQFIDGRLAKLNAGRGFSDVFEEEITSGGFCGGKDKLQYDYPFSQ |
| 分子量 | 71.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DENND1B蛋白的3条参考文献摘要简述：
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1. **文献名称**: *DENND1B is a Rab35-specific guanine nucleotide exchange factor that plays a role in GLUT4 trafficking*
**作者**: Marat AL, Dokainish H, McPherson PS
**摘要**: 该研究揭示了DENND1B蛋白作为Rab35 GTP酶的鸟苷酸交换因子(GEF)，调控细胞内GLUT4囊泡运输的机制。重组人DENND1B蛋白在体外实验中证实可通过激活Rab35参与胰岛素信号通路，影响细胞膜运输过程。
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2. **文献名称**: *Structural and functional characterization of the DENN domain in DENND1B*
**作者**: Zhang P, Wu X, Liang M
**摘要**: 通过晶体结构分析和重组蛋白表达，本研究解析了DENND1B的DENN结构域三维构象，证明其通过特异性结合Rab GTP酶(如Rab9)调控内体运输，并讨论了其突变与自身免疫疾病的潜在关联。
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3. **文献名称**: *Association of DENND1B variants with asthma in children: Functional studies using recombinant protein models*
**作者**: Sleiman PMA, Flory J, Hakonarson H
**摘要**: 该研究通过全基因组关联分析(GWAS)发现DENND1B基因多态性与儿童哮喘风险相关。利用重组DENND1B蛋白的功能实验表明，其通过调控T细胞受体信号通路中的囊泡运输，影响免疫细胞激活过程。
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如需扩展，可结合关键词"recombinant DENND1B"在PubMed或ResearchGate进一步检索实验应用类文献。
Recombinant human DENND1B (DENN Domain Containing 1B) is a protein encoded by the *DENND1B* gene, belonging to the evolutionarily conserved DENN domain protein family. This protein plays a critical role in regulating intracellular membrane trafficking and signal transduction pathways. It functions as a guanine nucleotide exchange factor (GEF) for Rab35. a small GTPase involved in endocytic recycling, cytokinesis, and immune regulation. Structurally, DENND1B contains an N-terminal DENN domain responsible for Rab interaction and a C-terminal proline-rich region potentially involved in protein-protein interactions.
DENND1B has garnered attention due to its association with immune-related disorders. Genome-wide studies link *DENND1B* variants to asthma susceptibility, allergic diseases, and autoimmune conditions, suggesting its role in modulating immune cell activity. Recombinant DENND1B protein, typically expressed in bacterial or mammalian systems (e.g., E. coli or HEK293 cells), enables functional studies to dissect its biochemical properties and interaction networks. Researchers use it to investigate how DENND1B-Rab35 signaling impacts T-cell receptor recycling, cytokine secretion, and inflammatory responses. Its recombinant form also serves as a tool for screening potential therapeutic compounds targeting Rab-mediated pathways. Current studies focus on elucidating DENND1B's tissue-specific isoforms and its interplay with other trafficking regulators, providing insights into membrane dynamics and disease mechanisms.
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