| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNASE1L1 |
| Uniprot No | P49184 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-302aa |
| 氨基酸序列 | AFRICAFNAQRLTLAKVAREQVMDTLVRILARCDIMVLQEVVDSSGSAIPLLLRELNRFDGSGPYSTLSSPQLGRSTYMETYVYFYRSHKTQVLSSYVYNDEDDVFAREPFVAQFSLPSNVLPSLVLVPLHTTPKAVEKELNALYDVFLEVSQHWQSKDVILLGDFNADCASLTKKRLDKLELRTEPGFHWVIADGEDTTVRASTHCTYDRVVLHGERCRSLLHTAAAFDFPTSFQLTEEEALNISDHYPVEVELKLSQAHSVQPLSLTVLLLLSLLSPQLCPAA |
| 分子量 | 57.09 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条与重组人DNASE1L1蛋白相关的研究文献摘要(基于近年研究整理)：
1. **文献名称**: "Recombinant human DNASE1L1 induces caspase-independent apoptosis through mitochondrial pathway"
**作者**: Lee S., et al. (2021)
**摘要**: 该研究利用大肠杆菌表达系统重组表达DNASE1L1蛋白，证明其可通过切割线粒体DNA引发caspase非依赖性细胞凋亡，揭示了其在调控线粒体功能中的新机制。
2. **文献名称**: "Structural insights into DNASE1L1’s endonuclease activity and calcium dependency"
**作者**: Zhang Y., et al. (2019)
**摘要**: 通过X射线晶体学解析了DNASE1L1的三维结构，阐明了其核酸内切酶活性位点及钙离子依赖性催化机制，为设计靶向该蛋白的抑制剂提供结构基础。
3. **文献名称**: "DNASE1L1 suppresses tumor metastasis by degrading neutrophil extracellular traps"
**作者**: Yang H., et al. (2020)
**摘要**: 研究显示重组DNASE1L1可通过降解中性粒细胞胞外诱捕网(NETs)，抑制肿瘤细胞迁移和转移，提出其在癌症免疫治疗中的潜在应用价值。
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**说明**：以上文献为示例性质，实际引用需通过PubMed或Web of Science检索具体论文。DNASE1L1研究多聚焦于其线粒体定位、细胞死亡调控及与NETs相关的炎症/癌症领域。
Recombinant human DNASE1L1 (Deoxyribonuclease 1-like 1) is a calcium/magnesium-dependent endonuclease belonging to the DNase I family. Unlike its homolog DNASE1. which primarily degrades extracellular DNA, DNASE1L1 is primarily localized to mitochondria and plays a role in hydrolyzing mitochondrial DNA (mtDNA) during apoptosis. It contains a conserved DNase I-like fold and requires Ca²⁺/Mg²⁺ ions for catalytic activity. The protein is encoded by the DNASE1L1 gene, spans approximately 10.9 kb on chromosome 11. and consists of 302 amino acids with a molecular weight of ~34 kDa.
DNASE1L1 is implicated in programmed cell death by facilitating mtDNA degradation, which disrupts mitochondrial integrity and promotes apoptotic signaling. It also participates in nuclear DNA fragmentation during late-stage apoptosis. Studies suggest its involvement in inflammation regulation by cleaving neutrophil extracellular traps (NETs) and mitigating autoimmune responses. Recombinant DNASE1L1 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies, structural analysis, and therapeutic exploration. Its unique mitochondrial targeting and DNA-cleaving properties make it a candidate for research in cancer, neurodegenerative diseases, and autoimmune disorders. Current investigations focus on its mechanistic role in mtDNA quality control, apoptosis-associated pathways, and potential as a biomarker or therapeutic target.
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