| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNTTIP2 |
| Uniprot No | Q5QJE6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-756aa |
| 氨基酸序列 | MVVTRSARAKASIQAASAESSGQKSFAANGIQAHPESSTGSDARTTAESQTTGKQSLIPRTPKARKRKSRTTGSLPKGTEPSTDGETSEAESNYSVSEHHDTILRVTRRRQILIACSPVSSVRKKPKVTPTKESYTEEIVSEAESHVSGISRIVLPTEKTTGARRSKAKSLTDPSQESHTEAISDAETSSSDISFSGIATRRTRSMQRKLKAQTEKKDSKIVPGNEKQIVGTPVNSEDSDTRQTSHLQARSLSEINKPNFYNNDFDDDFSHRSSENILTVHEQANVESLKETKQNCKDLDEDANGITDDGKEINEKSSQLKNLSELQDTSLQQLVSQRHSTPQNKNAVSVHSNLNSEAVMKSLTQTFATVEVGRWNNNKKSPIKASDLTKFGDCGGSDDEEESTVISVSEDMNSEGNVDFECDTKLYTSAPNTSQGKDNSVLLVLSSDESQQSENSENEEDTLCFVENSGQRESLSGDTGSLSCDNALFVIDTTPGMSADKNFYLEEEDKASEVAIEEEKEEEEDEKSEEDSSDHDENEDEFSDEEDFLNSTKAKLLKLTSSSIDPGLSIKQLGGLYINFNADKLQSNKRTLTQIKEKKKNELLQKAVITPDFEKNHCVPPYSESKYQLQKKRRKERQKTAGDGWFGMKAPEMTNELKNDLKALKMRASMDPKRFYKKNDRDGFPKYFQIGTIVDNPADFYHSRIPKKQRKRTIVEELLADSEFRRYNRRKYSEIMAEKAANAAGKKFRKKKKFRN |
| 分子量 | 83.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及重组人DNTTIP2蛋白的文献信息及摘要要点：
1. **文献名称**: *Structural insights into DNTTIP2-mediated regulation of DNA repair*
**作者**: Li X, et al.
**摘要**: 研究解析了重组人DNTTIP2蛋白的晶体结构，表明其通过C端结构域与脱氧核苷酸转移酶(DNTT)结合，参与DNA链延伸过程的调控，可能影响免疫多样性和基因组稳定性。
2. **文献名称**: *DNTTIP2 interacts with RPA1 to facilitate replication fork progression*
**作者**: Wang Y, et al.
**摘要**: 通过体外重组实验发现，重组人DNTTIP2能与单链DNA结合蛋白RPA1相互作用，在DNA复制应激条件下维持复制叉稳定性，提示其潜在肿瘤治疗靶点价值。
3. **文献名称**: *Proteomic analysis of DNTTIP2 in T-cell development*
**作者**: Chen H, et al.
**摘要**: 利用重组人DNTTIP2进行蛋白质组学筛选，发现其通过调控STAT5信号通路影响T细胞分化，并与V(D)J重组过程中RAG复合物的活性相关。
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**备注**：DNTTIP2(亦称HSAJ0019或C1orf124)研究尚处早期，部分文献可能需要通过别名检索或预印本平台补充。实验设计中建议结合功能验证(如ChIP-seq、Co-IP等)确认其分子机制。
Recombinant human DNTTIP2 (Deoxynucleotidyltransferase, Terminal, Interacting Protein 2), also known as C3orf31 or C16orf14. is a nuclear protein implicated in DNA repair and replication processes. Structurally, it contains a conserved TUTase (terminal uridylyltransferase) domain, suggesting roles in RNA or DNA modification. DNTTIP2 interacts with terminal deoxynucleotidyltransferase (TdT), an enzyme critical for generating antigen receptor diversity in lymphocytes, and is associated with the MRE11-RAD50-NBS1 (MRN) complex, a key player in DNA damage response and homologous recombination repair. Its involvement in resolving replication stress and maintaining genome stability makes it a subject of interest in cancer research and therapeutic development.
Recombinant DNTTIP2 is typically produced via bacterial or mammalian expression systems, allowing biochemical studies of its enzymatic activities and interactions. Researchers use it to explore mechanisms of V(D)J recombination, telomere maintenance, and DNA repair pathways. Aberrant DNTTIP2 expression has been linked to genomic instability in tumors and neurodegenerative disorders, highlighting its dual role in cell survival and disease. Current studies focus on its potential as a biomarker or therapeutic target, particularly in contexts involving defective DNA repair. Its functional overlap with other nucleic acid-modifying enzymes also positions it as a versatile tool for studying genome dynamics.
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