| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EBF3 |
| Uniprot No | Q9H4W6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-596aa |
| 氨基酸序列 | MFGIQENIPR GGTTMKEEPL GSGMNPVRSW MHTAGVVDAN TAAQSGVGLA RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV DFVEKEKEPN NEKTNNGIHY KLQLLYSNGV RTEQDLYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS EGTAPSYLEN ATPCIKAISP SEGWTTGGAT VIIIGDNFFD GLQVVFGTML VWSELITPHA IRVQTPPRHI PGVVEVTLSY KSKQFCKGAP GRFVYTALNE PTIDYGFQRL QKVIPRHPGD PERLPKEVLL KRAADLVEAL YGMPHNNQEI ILKRAADIAE ALYSVPRNHN QIPTLGNNPA HTGMMGVNSF SSQLAVNVSE TSQANDQVGY SRNTSSVSPR GYVPSSTPQQ SNYNTVSTSM NGYGSGAMAS LGVPGSPGFL NGSSANSPYG IVPSSPTMAA SSVTLPSNCS STHGIFSFSP ANVISAVKQK SAFAPVVRPQ ASPPPSCTSA NGNGLQAMSG LVVPPM |
| 分子量 | 64.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EBF3蛋白的3篇代表性参考文献(根据领域研究概况概括，非真实文献)：
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1. **《Expression and Functional Characterization of Recombinant Human EBF3 in Neural Stem Cells》**
*作者：Chen L et al.*
**摘要**：本研究通过哺乳动物表达系统成功重组表达了人EBF3蛋白，纯化后验证其对神经干细胞分化的调控作用。实验表明，EBF3通过结合特定DNA序列激活下游基因，促进神经前体细胞向成熟神经元分化。
2. **《Structural Analysis of EBF3 Reveals Key Domains for DNA Binding and Dimerization》**
*作者：Wang Y et al.*
**摘要**：该研究利用大肠杆菌系统表达并纯化重组人EBF3蛋白，通过X射线晶体学解析其三维结构，揭示了N端锌指结构域和C端二聚化结构域对EBF3的DNA结合能力和功能调控至关重要。
3. **《EBF3 Suppresses Tumor Metastasis via Epigenetic Regulation in Colorectal Cancer》**
*作者：Kim S et al.*
**摘要**：研究者通过昆虫细胞表达系统获得高纯度重组EBF3蛋白，发现其通过招募组蛋白去乙酰化酶(HDACs)修饰染色质，抑制结直肠癌侵袭转移相关基因表达，为EBF3的抑癌机制提供依据。
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**注**：以上文献为示例性概括，实际研究中需根据具体论文调整信息。建议通过PubMed或Web of Science以关键词“EBF3 recombinant protein”或“EBF3 expression”检索真实文献。
Recombinant human EBF3 (Early B-Cell Factor 3) is a transcription factor belonging to the Collier/Olf/EBF (COE) family. EBF3 plays a critical role in neuronal development, differentiation, and tumor suppression by regulating gene expression through binding to specific DNA motifs (E-box sequences). It contains a highly conserved DNA-binding domain and a atypical zinc knuckle motif, enabling its interaction with co-regulators. Dysregulation of EBF3 is linked to neurodevelopmental disorders, such as autism and intellectual disability, and its loss-of-function mutations are observed in various cancers, including gliomas and lung adenocarcinomas.
Produced via recombinant DNA technology—typically in Escherichia coli or mammalian expression systems—the purified protein retains native structural and functional properties. Its recombinant form enables studies on molecular mechanisms, including chromatin remodeling, cellular signaling pathways, and epigenetic regulation. Researchers utilize it in vitro/in vivo assays, protein-DNA interaction studies, and as a tool for exploring therapeutic targets in neurological diseases and oncology.
Current research focuses on elucidating its role in neural crest cell migration, synaptic plasticity, and tumor microenvironment modulation. Challenges include understanding tissue-specific isoforms and post-translational modifications affecting its activity. Recombinant EBF3 serves as a vital reagent for bridging molecular biology with clinical applications.
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