关键信息
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基因名
TNNI2
- 应用
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别名
TNNI2；Troponin I. fast skeletal muscle
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48788
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表达区间
1-182aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGDEEKRNRAITARRQHLKSVMLQIAATEL EKEESRREAEKQNYLAEHCPPLHIPGSMSEVQELCKQLHAKIDAAEEEKY DMEVRVQKTSKELEDMNQKLFDLRGKFKRPPLRRVRMSADAMLKALLGSK HKVCMDLRANLKQVKKEDTEKERDLRDVGDWRKNIEEKSGMEGRKKMFES ES
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分子量
24 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of TNNI2, or troponin I type 2, has gained significant traction due to its critical role in cardiac and skeletal muscle contraction regulation. TNNI2 is a key component of the troponin complex, which, together with tropomyosin, regulates the interaction between actin and myosin in muscle fibers. Mutations or dysregulations in TNNI2 have been associated with various myopathies and cardiac diseases, emphasizing the importance of understanding its structure and function. The recombinant production of TNNI2 allows researchers to study its properties in detail, providing insights into muscle physiology and pathophysiology. Furthermore, recombinant TNNI2 can be used to investigate the effects of specific mutations on protein function, aiding in the identification of therapeutic targets for muscle-related disorders. This research not only enhances our comprehension of muscle contraction mechanics but also has potential implications for developing treatments for conditions linked to TNNI2 mutations, such as familial hypertrophic cardiomyopathy and other myopathies. As such, the investigation of TNNI2 recombinant protein represents a vital area of research with broad biomedical applications.
