| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EML1 |
| Uniprot No | O00423 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-815aa |
| 氨基酸序列 | MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLMQTLPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKSNIKRTSSSERVSPGGRRESNGDSRGNRNRTGSTSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSLEAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGTNLCAVDDSNDHVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKFVLCVTFSENGDTITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGNYQKLRKTEIPEQFGPIRTVAEGKGDVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAINASKSQFLTCGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTGTKDLVTVHTDGNEQLSVMRYSPDGNFLAIGSHDNCIYIYGVSDNGRKYTRVGKCSGHSSFITHLDWSVNSQFLVSNSGDYEILYWVPSACKQVVSVETTRDIEWATYTCTLGFHVFGVWPEGSDGTDINAVCRAHEKKLLSTGDDFGKVHLFSYPCSQFRAPSHIYGGHSSHVTNVDFLCEDSHLISTGGKDTSIMQWRVI |
| 分子量 | 115.39 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EML1蛋白的3条示例文献(内容为模拟概括，具体文献需通过数据库检索确认)：
1. **文献名称**：Crystal structure of human EML1 reveals evolutionary conserved microtubule-binding mode
**作者**：Zhang Y, et al.
**摘要**：解析了重组人EML1蛋白的晶体结构，揭示其N端WD40重复结构域与微管结合的分子机制，并探讨其在细胞分裂中的作用。
2. **文献名称**：Functional characterization of recombinant EML1 in neurodevelopmental disorders
**作者**：Kessler MD, et al.
**摘要**：通过体外表达重组人EML1蛋白，发现其突变导致神经元迁移异常，表明EML1功能缺失与皮层发育畸形相关。
3. **文献名称**：EML1 modulates microtubule dynamics via MAPK signaling pathway in cancer cells
**作者**：Li H, Wang J.
**摘要**：利用重组人EML1蛋白的过表达/敲低模型，证明其通过调控ERK磷酸化影响微管稳定性，从而促进肿瘤细胞侵袭转移。
注：以上为示例性内容，实际文献建议通过PubMed/Google Scholar以“recombinant human EML1”或“EML1 protein function”为关键词检索，重点关注近十年的结构生物学、细胞生物学相关研究。
Recombinant human EML1 protein is a genetically engineered version of the Echinoderm Microtubule-Associated Protein-Like 1 (EML1), which belongs to the conserved EMAP/EML family. EML1 is involved in microtubule dynamics, cell division, and neural development. It contains tandem WD40-repeat domains and a hydrophobic EML-specific motif, enabling interactions with microtubules and associated proteins. Dysregulation of EML1 has been linked to neurodevelopmental disorders, particularly periventricular nodular heterotopia (PVNH), a brain malformation caused by defective neuronal migration during corticogenesis. Studies using animal models and patient-derived cells suggest EML1’s critical role in regulating microtubule stability and cytoskeletal organization during cortical layer formation.
Recombinant EML1 is typically expressed in prokaryotic (e.g., E. coli) or eukaryotic systems (e.g., mammalian cells) to study its structure-function relationships. Researchers employ it for in vitro assays, such as microtubule-binding experiments, co-immunoprecipitation studies, or drug screening for potential therapies targeting EML1-related pathways. Its recombinant form enables high-purity, scalable production for structural analyses (e.g., X-ray crystallography) and functional characterization. Current research focuses on elucidating how EML1 mutations disrupt microtubule-mediated processes in neurodevelopment and exploring therapeutic strategies to mitigate these effects. The protein’s role in cancer progression, particularly in microtubule inhibitor resistance, is also an emerging area of investigation.
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