| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTR4 |
| Uniprot No | Q13639 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-388aa |
| 氨基酸序列 | MDKLDANVSSEEGFGSVEKVVLLTFLSTVILMAILGNLLVMVAVCWDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAIELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIALMLGGCWVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVTAKEHAHQIQMLQRAGASSESRPQSADQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTNIVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTTINGSTHVLRDAVECGGQWESQCHPPATSPLVAAQPSDT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HTR4重组蛋白的3篇参考文献示例(内容基于真实研究领域，但文献标题和作者为模拟概括，供参考)：
1. **文献名称**: "Functional Characterization of Recombinant Human 5-HT4 Receptors in HEK293 Cells"
**作者**: Smith A, et al.
**摘要**: 本研究在HEK293细胞中表达了重组人源HTR4受体，通过cAMP信号通路分析其激活机制，证实了该受体对5-羟色胺及特异性激动剂的高亲和力，为药物筛选提供了模型。
2. **文献名称**: "Cryo-EM Structure of the 5-HT4 Receptor Bound to an Agonist"
**作者**: Zhang L, et al.
**摘要**: 利用冷冻电镜技术解析了重组HTR4蛋白与激动剂结合的近原子分辨率结构，揭示了其跨膜结构域的构象变化及G蛋白偶联的关键位点。
3. **文献名称**: "High-Yield Expression and Purification of HTR4 in Insect Cells for Biophysical Studies"
**作者**: Tanaka K, et al.
**摘要**: 报道了一种在昆虫细胞系统中高效表达并纯化功能性HTR4重组蛋白的方法，验证了其药理学活性及稳定性，为后续结构-功能研究奠定基础。
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**备注**：以上文献信息为示例性质，具体研究需通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。实际文献可能更侧重HTR4的病理生理机制或药物开发应用。
**Background of HTR4 Recombinant Protein**
The 5-Hydroxytryptamine receptor 4 (HTR4), a member of the G protein-coupled receptor (GPCR) family, plays a critical role in mediating the effects of serotonin (5-HT), a neurotransmitter involved in regulating mood, cognition, and gastrointestinal motility. HTR4 is encoded by the *HTR4* gene in humans, located on chromosome 5q32. Unlike other serotonin receptors, HTR4 primarily couples to Gαs proteins, activating adenylate cyclase and increasing intracellular cAMP levels, which modulates downstream signaling pathways.
Recombinant HTR4 protein is engineered in vitro using expression systems (e.g., HEK293 or CHO cells) to produce functional, purified receptors for research and drug development. These systems enable precise control over post-translational modifications, ensuring structural and functional fidelity. The recombinant protein often includes tags (e.g., His or FLAG) to facilitate purification and detection.
HTR4 is widely studied for its roles in neurological and gastrointestinal disorders. It influences neurotransmitter release in the brain, linking it to conditions like depression, anxiety, and Alzheimer’s disease. In the gut, HTR4 regulates peristalsis and secretion, making it a target for irritable bowel syndrome (IBS) therapies. Recombinant HTR4 aids in screening agonists/antagonists, studying receptor-ligand interactions, and developing targeted drugs.
Challenges in working with HTR4 include its conformational instability and low solubility in vitro. Advances in membrane protein stabilization techniques (e.g., nanodiscs or detergent optimization) have improved structural studies, including cryo-EM and X-ray crystallography. Recombinant HTR4 remains pivotal for unraveling serotonin signaling mechanisms and designing therapeutics with enhanced specificity and reduced off-target effects.
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