Recombinant Human FAM45A Protein

Recombinant human FAM45A protein is a product of the FAM45A gene, which encodes a conserved but poorly characterized protein across eukaryotic species. Although the exact biological functions of FAM45A remain elusive, bioinformatic analyses suggest it may play roles in cellular stress responses, apoptosis regulation, or nucleic acid binding due to predicted structural motifs like coiled-coil domains. The protein is predominantly localized in the cytoplasm and nucleus, as observed in preliminary cellular studies. Its recombinant version is typically produced using expression systems such as *E. coli* or mammalian cells, enabling structural and functional investigations. Research interest in FAM45A has recently grown due to potential disease associations: transcriptomic data links its dysregulation to cancers (e.g., hepatocellular carcinoma) and neurological disorders, while epigenetic studies suggest possible involvement in oxidative stress pathways. However, mechanistic insights are limited by the lack of specific antibodies and validated interaction partners. Current applications focus on using recombinant FAM45A protein for antibody generation, protein-protein interaction screening, and *in vitro* assays to delineate its molecular functions. Challenges persist in establishing standardized purification protocols due to the protein’s unknown post-translational modifications and solubility characteristics. Collaborative efforts in structural biology (e.g., crystallography) and CRISPR-based functional genomics are expected to accelerate the characterization of this enigmatic protein.