| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM71A |
| Uniprot No | Q8IYT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-594aa |
| 氨基酸序列 | MNADFLLPYYTAQSGSSMSMFDTTMGKLQRQLYKGEYDIFKYAPIFESDFIQITKRGEVIDVHNRVRMVTMGIARTSPILPLPDVMLLARPATGCEEYAGHGQATKRKKRKAAKNLELTRLLPLRFVRISVQDHEKQQLRLKFATGRSCYLQLCPALDTRDDLFAYWEKLIYLLRPPMESNSSTCGIPAEDMMWMPVFQEDRRSLGAVNLQGKGDQDQVSIQSLHMVSEVCGATSAAYAGGEGLQNDFNKPTNVLNASIPKTSTELAEEPATGGIKEAAAAGAAAGAATGTVAGALSVAAANSAPGQVSAAIAGAATIDAGGNKGNMALAGTASMAPNSTKVAVAGAAGKSSEHVSSASMSLSREGSVSLAIAGVVLTSRTAAEADMDAAAGPPVSTRQSKSSLSGQHGRERTQASAEGCKEGRERREKDRALGRSSHRRRTGESRHKTRGDKIAQKSSSRSSFSHRANRDDKKEKGCGNPGSSRHRDSHKGVSHTPISKESRTSHKSGRSLWTTSSGSSKGLGRVSSFLRNVRANLTTKVVGTPHGRDVDVMAKMAERSTNVAIAETAEGGQGLEMVGSMTPDIMETVTFEAH |
| 分子量 | 89.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM71A蛋白的假设性参考文献示例，基于当前研究方向的合理推断：
1. **文献名称**: *FAM71A is a component of the sperm fibrous sheath and is essential for male fertility in mice*
**作者**: Chen, H.C., et al.
**摘要**: 研究发现FAM71A是精子纤维鞘的关键结构蛋白，通过重组蛋白技术在小鼠模型中证实其缺失导致精子运动缺陷和雄性不育，揭示了其在生殖系统中的功能性作用。
2. **文献名称**: *Recombinant Expression and Purification of Human FAM71A in a Prokaryotic System*
**作者**: Zhang, Y., et al.
**摘要**: 本文成功在大肠杆菌中高效表达并纯化重组人FAM71A蛋白，优化了可溶性表达条件，为后续抗体开发及功能研究提供了材料基础。
3. **文献名称**: *FAM71A Interacts with PI3K Signaling Pathway to Modulate Cell Migration*
**作者**: Tanaka, K., et al.
**摘要**: 利用重组人FAM71A处理体外培养的肿瘤细胞，发现其通过调控PI3K通路抑制细胞迁移，提示其潜在肿瘤抑制功能。
4. **文献名称**: *Structural and Biochemical Characterization of Recombinant Human FAM71A*
**作者**: Gupta, S., et al.
**摘要**: 通过冷冻电镜和酶活实验解析重组人FAM71A的三维结构，揭示了其可能的磷酸水解酶活性及底物结合域，为机制研究提供结构依据。
**注**：上述文献为模拟示例，实际研究中可能需查询具体数据库(如PubMed)以获取真实发表论文。若需真实文献，建议使用关键词“FAM71A recombinant”或“FAM71A protein”在学术引擎中检索。
**Background of Recombinant Human FAM71A Protein**
The FAM71A (Family with Sequence Similarity 71 Member A) protein is a relatively understudied member of the FAM71 family, encoded by the *FAM71A* gene located on human chromosome 1. Emerging evidence suggests its potential role in male reproductive biology, particularly in spermatogenesis and sperm function. FAM71A is predominantly expressed in the testis and has been associated with sperm motility and structural integrity, though its precise molecular mechanisms remain unclear.
Recombinant human FAM71A protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its production involves cloning the *FAM71A* coding sequence into expression vectors, followed by purification via affinity chromatography. This recombinant form facilitates investigations into its interactions with cellular components, post-translational modifications, and potential binding partners.
Recent studies highlight FAM71A’s potential involvement in fertility disorders, positioning it as a candidate biomarker or therapeutic target. However, gaps persist in understanding its physiological roles beyond reproduction, including possible links to cellular signaling or stress responses. The development of recombinant FAM71A protein provides a critical tool for unraveling its biological significance and advancing applications in reproductive health research and diagnostics. Further studies are needed to map its interactome and validate its functional pathways.
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