| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM71B |
| Uniprot No | Q8TC56 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605aa |
| 氨基酸序列 | MSNESCLPYYTAHSYSSMSAFKTSMGDLQRQLYNRGEYNIFKYAPMFESNFIQINKKGEVIDVHNRVRMVTVGIVCTSPILPLPDVMVLAQPTKICEQHVRWGRFAKGRGRRPVKTLELTRLLPLKFVKISIHDHEKQQLRLKLATGRTFYLQLCPSSDTREDLFCYWEKLVYLLRPPVESYCSTPTLLSGDAPPEDNKSLVAAELHREGDQSETGLYKPCDVSAATSSAYAGGEGIQHASHGTASAASPSTSTPGAAEGGAARTAGGMAVAGTATGPRTDVAIAGAAMSPATGAMSIATTKSAGPGQVTTALAGAAIKNPGENESSKSMAGAANISSEGISLALVGAASTSLEGTSTSMAGAASLSQDSSLSAAFAGSITTSKCAAERTEGPAVGPLISTLQSEGYMSERDGSQKVSQPSAEVWNENKERREKKDRHPSRKSSHHRKAGESHRRRAGDKNQKASSHRSASGHKNTRDDKKEKGYSNVRGKRHGSSRKSSTHSSTKKESRTTQELGKNQSASSTGALQKKASKISSFLRSLRVTPGSKTRVTSHDREVDIVAKMVEKQNIEAKVEKAQGGQELEMISGTMTSEKTEMIVFETKSI |
| 分子量 | 91.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于FAM71B蛋白现有研究背景模拟的参考文献示例(非真实文献)：
1. **《FAM71B在男性生殖中的功能鉴定》**
*作者：Zhang, L. et al.*
摘要：研究通过基因敲除模型发现，FAM71B蛋白参与精子鞭毛结构形成，其缺失导致小鼠精子运动能力下降，提示其在男性不育症中的潜在作用。
2. **《重组人FAM71B蛋白的体外表达与纯化》**
*作者：Chen, W. & Li, Y.*
摘要：首次报道在HEK293细胞中成功表达可溶性重组FAM71B蛋白，优化纯化方法后获得高纯度蛋白，为后续功能研究提供材料基础。
3. **《FAM71B与前列腺癌细胞迁移的关联研究》**
*作者：Kim, S. et al.*
摘要：通过蛋白质组学分析发现，FAM71B在前列腺癌转移组织中高表达，体外实验证实其过表达可促进肿瘤细胞侵袭能力，提示其作为癌症标志物的可能性。
4. **《FAM71B的磷酸化修饰调控机制》**
*作者：Gupta, R. & Park, J.H.*
摘要：研究发现FAM71B在睾丸组织中存在特异性的丝氨酸磷酸化修饰，并通过质谱鉴定关键修饰位点，揭示翻译后修饰可能调节其生物学功能。
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注：以上文献为模拟内容，实际研究请通过PubMed、Web of Science等学术平台检索。真实研究方向可能集中在精子发生、鞭毛组装或癌症相关领域。
Recombinant human FAM71B protein is derived from the FAM71B (Family with Sequence Similarity 71 Member B) gene, which encodes a poorly characterized protein predominantly expressed in the male reproductive system, particularly in the testis. Emerging studies suggest its potential role in spermatogenesis or sperm function, though its precise molecular mechanisms remain unclear. FAM71B is conserved across species, indicating possible functional importance, but its structure and interaction partners are largely unexplored.
The recombinant form is typically produced using heterologous expression systems like bacteria or mammalian cells, enabling large-scale purification for functional studies. This approach overcomes challenges associated with isolating native FAM71B from biological tissues. Recombinant FAM71B facilitates investigations into its biochemical properties, subcellular localization, and involvement in cellular processes. Notably, altered FAM71B expression has been tentatively linked to male infertility, positioning it as a potential biomarker or therapeutic target in reproductive disorders.
Current research remains preliminary, focusing on validating its physiological relevance. Further studies are needed to elucidate its role in fertility, post-translational modifications, and pathways it may regulate. The development of recombinant FAM71B tools accelerates mechanistic studies, opening avenues for understanding its contribution to human health and disease.
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