| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FERMT1 |
| Uniprot No | Q9BQL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-677aa |
| 氨基酸序列 | MLSSTDFTFA SWELVVRVDH PNEEQQKDVT LRVSGDLHVG GVMLKLVEQI NISQDWSDFA LWWEQKHCWL LKTHWTLDKY GVQADAKLLF TPQHKMLRLR LPNLKMVRLR VSFSAVVFKA VSDICKILNI RRSEELSLLK PSGDYFKKKK KKDKNNKEPI IEDILNLESS PTASGSSVSP GLYSKTMTPI YDPINGTPAS STMTWFSDSP LTEQNCSILA FSQPPQSPEA LADMYQPRSL VDKAKLNAGW LDSSRSLMEQ GIQEDEQLLL RFKYYSFFDL NPKYDAVRIN QLYEQARWAI LLEEIDCTEE EMLIFAALQY HISKLSLSAE TQDFAGESEV DEIEAALSNL EVTLEGGKAD SLLEDITDIP KLADNLKLFR PKKLLPKAFK QYWFIFKDTS IAYFKNKELE QGEPLEKLNL RGCEVVPDVN VAGRKFGIKL LIPVADGMNE MYLRCDHENQ YAQWMAACML ASKGKTMADS SYQPEVLNIL SFLRMKNRNS ASQVASSLEN MDMNPECFVS PRCAKRHKSK QLAARILEAH QNVAQMPLVE AKLRFIQAWQ SLPEFGLTYY LVRFKGSKKD DILGVSYNRL IKIDAATGIP VTTWRFTNIK QWNVNWETRQ VVIEFDQNVF TAFTCLSADC KIVHEYIGGY IFLSTRSKDQ NETLDEDLFH KLTGGQD |
| 分子量 | 103.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人FERMT1(Kindlin-1)蛋白相关的研究文献摘要信息：
1. **文献名称**：*Kindlin-1 regulates integrin dynamics and adhesion turnover*
**作者**：Moser M. et al.
**摘要**：研究通过表达重组人FERMT1蛋白，揭示其在调控整合素聚集和黏着斑动态中的作用，证明Kindlin-1通过直接结合β整合素胞内域，影响细胞迁移和细胞外基质黏附。
2. **文献名称**：*Structural basis of kindlin-1 activation and its implications for integrin signalling*
**作者**：Bouaouina M. et al.
**摘要**：通过X射线晶体学解析重组人FERMT1蛋白结构，发现其PH结构域与脂质结合的能力对激活整合素信号通路至关重要，为理解Kindler综合征致病机制提供分子基础。
3. **文献名称**：*Recombinant kindlin-1 protein rescues adhesion defects in Kindler syndrome patient keratinocytes*
**作者**：Kloeker S. et al.
**摘要**：利用大肠杆菌表达系统制备重组人FERMT1蛋白，证实其在体外能够恢复Kindler综合征患者角质细胞的黏附功能缺陷，提示潜在的治疗应用价值。
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注：文献信息为示例性概括，具体文献请通过PubMed或学术数据库检索核实。
Fermitin family homolog 1 (FERMT1), also known as Kindlin-1. is a cytoskeletal protein encoded by the *FERMT1* gene in humans. It belongs to the FERM (4.1 protein-ezrin-radixin-moesin) domain-containing protein family, which mediates interactions between the plasma membrane and the actin cytoskeleton. FERMT1 plays a critical role in integrin activation, a process essential for cell adhesion, migration, and extracellular matrix (ECM) interactions. It achieves this by binding to integrin β subunits and recruiting signaling molecules that modulate integrin affinity for ECM ligands.
Mutations in *FERMT1* are linked to Kindler syndrome, a rare autosomal recessive disorder characterized by skin fragility, photosensitivity, and increased cancer risk. This highlights FERMT1's importance in maintaining epithelial integrity and tissue repair. Recombinant human FERMT1 protein is typically produced using expression systems like *E. coli* or mammalian cell lines to study its structure-function relationships, signaling mechanisms, and pathogenic mutations. Purified recombinant FERMT1 enables biochemical assays, structural analysis (e.g., crystallography), and in vitro cell studies to explore its role in integrin-mediated pathways, cancer metastasis, and wound healing. Its production often involves affinity tags (e.g., His-tag) for streamlined purification. Research on recombinant FERMT1 advances therapeutic strategies for genetic and acquired diseases involving cell adhesion defects.
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