| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLJ40125 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-340aa |
| 氨基酸序列 | MEDAHCTWLSLPGLPPGWALFAVLDGHGGARAARFGARHLPGHVLQELGPEPSEPEGVREALRRAFLSADERLRSLWPRVETGGCTAVVLLVSPRFLYLAHCGDSRAVLSRAGAVAFSTEDHRPLRPRERERIHAAGGTIRRRRVEGSLAVSRALGDFTYKEAPGRPPELQLVSAEPEVAALARQAEDEFMLLASDGVWDTVSGAALAGLVASRLRLGLAPELLCAQLLDTCLCKVLGAWRGTFGAWCSRGREPRGFGEEGFDREARVKLAKEGIGLKRRAWPEVGRAKIQGRSLRDALRHGRVSGRDLRERAWSFGKGRFWVVGAGPEVLISAGRWKSR |
| 分子量 | 63.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FLJ40125蛋白的模拟参考文献示例(注：文献为假设性概括，需通过学术数据库验证真实性)：
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1. **文献名称**：**"Expression and Functional Characterization of Recombinant Human FLJ40125 in Mammalian Cells"**
**作者**：Smith JL, et al.
**摘要**：本文报道了通过哺乳动物表达系统成功制备重组人FLJ40125蛋白，并验证其参与泛素-蛋白酶体通路的调控功能。Western blot和质谱分析表明该蛋白与癌症相关蛋白TP53存在相互作用。
2. **文献名称**：**"Structural Insights into FLJ40125: A Novel Protein with Kinase-like Domains"**
**作者**：Chen X, et al.
**摘要**：通过X射线晶体学解析了重组FLJ40125的三维结构，揭示了其激酶样结构域的特征，并发现其可能在细胞周期调控中通过磷酸化作用激活下游信号分子。
3. **文献名称**：**"FLJ40125 Deficiency Attenuates Oxidative Stress in Mouse Models of Metabolic Syndrome"**
**作者**：Tanaka K, et al.
**摘要**：研究表明，FLJ40125敲除小鼠表现出对高脂饮食诱导的氧化应激抵抗，提示该蛋白可能通过调节线粒体活性氧(ROS)的生成影响代谢疾病进程。
4. **文献名称**：**"Proteomic Analysis of FLJ40125-Interacting Proteins in Human Hepatocellular Carcinoma"**
**作者**：Li R, et al.
**摘要**：利用免疫共沉淀结合质谱技术筛选出FLJ40125在肝癌细胞中的互作蛋白网络，包括DNA修复蛋白BRCA1及凋亡相关因子，提示其潜在促癌作用。
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**建议**：实际研究中，请通过PubMed、Web of Science等平台，以**“FLJ40125 protein”**或**“FLJ40125 recombinant”**为关键词检索最新文献，并优先选择近五年内发表的论文以确保信息时效性。此外，可参考UniProt数据库条目(如UniProt ID：Q8NXXX)获取该蛋白的功能注释。
Recombinant human FLJ40125 protein is a genetically engineered protein derived from the FLJ40125 gene, which is located on human chromosome 17q21.2. Initially identified through genomic sequencing projects, FLJ40125 is categorized as a hypothetical protein with limited functional characterization. Bioinformatics analyses suggest it may belong to the tyrosine kinase family due to conserved structural domains, though its exact physiological role remains unclear. Studies indicate potential involvement in cellular signaling pathways, possibly interacting with proteins related to Wnt signaling or MAPK cascades, but experimental validation is lacking.
The recombinant form is typically produced in heterologous expression systems (e.g., E. coli, HEK293 cells) to enable functional and structural studies. Its production facilitates investigations into its biochemical properties, substrate interactions, and potential roles in diseases. Some evidence links FLJ40125 overexpression to cancer progression, particularly breast and lung cancers, suggesting it might serve as a biomarker or therapeutic target. However, research remains preliminary, emphasizing the need for deeper mechanistic insights.
Current applications include antibody development, protein-protein interaction assays, and exploratory studies in cell proliferation/apoptosis models. Challenges include optimizing protein stability and resolving discrepancies between in silico predictions and experimental outcomes. Overall, recombinant FLJ40125 represents a tool to decode the functional relevance of understudied proteins in human biology and disease.
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