| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCARA5 |
| Uniprot No | Q6ZMJ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-495aa |
| 氨基酸序列 | MENKAMYLHTVSDCDTSSICEDSFDGRSLSKLNLCEDGPCHKRRASICCTQLGSLSALKHAVLGLYLLVFLILVGIFILAVSRPRSSPDDLKALTRNVNRLNESFRDLQLRLLQAPLQADLTEQVWKVQDALQNQSDSLLALAGAVQRLEGALWGLQAQAVQTEQAVALLRDRTGQQSDTAQLELYQLQVESNSSQLLLRRHAGLLDGLARRVGILGEELADVGGVLRGLNHSLSYDVALHRTRLQDLRVLVSNASEDTRRLRLAHVGMELQLKQELAMLNAVTEDLRLKDWEHSIALRNISLAKGPPGPKGDQGDEGKEGRPGIPGLPGLRGLPGERGTPGLPGPKGDDGKLGATGPMGMRGFKGDRGPKGEKGEKGDRAGDASGVEAPMMIRLVNGSGPHEGRVEVYHDRRWGTVCDDGWDKKDGDVVCRMLGFRGVEEVYRTARFGQGTGRIWMDDVACKGTEETIFRCSFSKWGVTNCGHAEDASVTCNRH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCARA5重组蛋白的示例参考文献(内容为示例，建议通过学术数据库核实具体研究)：
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1. **文献名称**：*Structural and functional characterization of SCARA5 as a cell adhesion receptor*
**作者**：Li, X., et al.
**摘要**：研究通过重组表达SCARA5蛋白，解析其胞外结构域的三维构象，揭示其通过胶原样结构域介导细胞间黏附的分子机制，并验证其在胚胎发育中的潜在作用。
2. **文献名称**：*SCARA5 inhibits tumor metastasis via suppressing Wnt/β-catenin signaling in hepatocellular carcinoma*
**作者**：Wang, Y., et al.
**摘要**：利用重组SCARA5蛋白进行体外实验，证明其通过结合Wnt配体抑制β-catenin信号通路，从而抑制肝癌细胞迁移和侵袭，提出其作为肿瘤抑制因子的功能。
3. **文献名称**：*Recombinant SCARA5 binds to bacterial surface proteins and modulates innate immune response*
**作者**：Zhang, H., et al.
**摘要**：研究重组SCARA5与革兰氏阳性菌表面蛋白的相互作用，发现其通过激活巨噬细胞NF-κB通路增强抗菌免疫反应，为感染性疾病治疗提供新靶点。
4. **文献名称**：*Glycosylation analysis of recombinant SCARA5 and its role in ligand recognition*
**作者**：Chen, L., et al.
**摘要**：通过质谱分析重组SCARA5的糖基化修饰位点，证实其糖链结构对配体(如胶原IV)的结合特异性至关重要，并影响下游细胞信号传导。
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建议通过PubMed或Web of Science检索关键词“SCARA5 recombinant protein”获取真实文献。
**Background of SCARA5 Recombinant Protein**
SCARA5 (Scavenger Receptor Class A Member 5) is a transmembrane glycoprotein belonging to the class A scavenger receptor family, known for its roles in innate immunity, cell adhesion, and homeostasis. Structurally, it contains a short cytoplasmic tail, a transmembrane domain, and an extracellular region with collagen-like and scavenger receptor cysteine-rich (SRCR) domains. These domains enable SCARA5 to interact with diverse ligands, including pathogen-associated molecular patterns (PAMPs), extracellular matrix components (e.g., laminin, collagen), and apoptotic cells, suggesting its involvement in immune surveillance and tissue remodeling.
SCARA5 is highly expressed in epithelial and endothelial cells, particularly in the liver, kidney, and placenta. Studies highlight its dual role in physiological and pathological contexts. In normal tissues, it supports cell adhesion and migration by binding to laminin, facilitating tissue integrity. However, in cancer, SCARA5 often exhibits downregulated expression, correlating with tumor progression and metastasis in hepatocellular carcinoma, breast cancer, and glioblastoma. This tumor-suppressive function is linked to its ability to inhibit Wnt/β-catenin and PI3K/Akt signaling pathways, reducing cell proliferation and invasion.
Recombinant SCARA5 protein is produced using mammalian or insect cell systems to ensure proper post-translational modifications, such as glycosylation, critical for ligand-binding activity. Purified SCARA5 is utilized in functional studies to explore its interactions, signaling mechanisms, and therapeutic potential. For instance, it serves as a tool to develop targeted therapies, including monoclonal antibodies or small-molecule inhibitors, to restore SCARA5 function in cancers or modulate immune responses. Additionally, its role in pathogen recognition positions it as a candidate for vaccine adjuvants or antimicrobial strategies.
Overall, SCARA5 recombinant protein bridges structural biology and translational research, offering insights into its multifaceted roles and paving the way for novel diagnostic and therapeutic applications.
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