关键信息
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基因名
USP14
- 应用
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别名
USP14；TGT；Ubiquitin carboxyl-terminal hydrolase 14
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54578
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表达区间
2-494aa
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氨基酸序列
PLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGG TLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAM ELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQY ITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDAN ECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEF ETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQ SPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPL MLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKY EPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFD DDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ
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分子量
57 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
USP14 (Ubiquitin-specific protease 14) is a deubiquitinating enzyme that plays a critical role in the regulation of protein turnover and cellular homeostasis by removing ubiquitin moieties from proteasomal substrates. Its involvement in various cellular processes, including protein quality control, signal transduction, and stress responses, highlights its significance in maintaining cellular functions. Research has shown that dysregulation of USP14 is associated with several neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease, where the accumulation of misfolded or damaged proteins occurs. Understanding the functional mechanisms of USP14 may provide insights into therapeutic strategies for these conditions. The study of recombinant USP14 protein has garnered attention for its potential applications in drug discovery and the development of proteasome-modulating therapies. By investigating the structure, function, and interaction of USP14 with other cellular components, researchers aim to elucidate its role in ubiquitin-proteasome system regulation and its impact on cellular health. This involves recombinant expression and purification techniques to produce active USP14, allowing for in-depth biochemical and biophysical analyses. Therefore, the exploration of USP14 as a therapeutic target may pave the way for novel approaches in treating neurodegenerative disorders and advancing our understanding of protein homeostasis in human health.
