关键信息
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基因名
ATP5I
- 应用
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别名
ATP5K; ATPase subunit e; F1F0 ATP synthase murine e subunit; MGC12532; mitochondrial
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P56385
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表达区间
1-69aa
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氨基酸序列
MVPPVQVSPLIKLGRYSALFLGVAYGATRYNYLKPRAEEERRIAAEEKKKQDELKRIARELAEDDSILK
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分子量
33.33 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ATP5I, also known as ATP synthase subunit 5I, is a crucial component of the mitochondrial ATP synthase complex, which is essential for oxidative phosphorylation and ATP production in eukaryotic cells. Research into ATP5I has gained traction due to its potential involvement in various metabolic disorders and mitochondrial dysfunction, which are linked to a range of diseases, including neurodegenerative disorders, cancer, and cardiovascular diseases. Recent studies have highlighted ATP5I's role not only in ATP production but also in regulating mitochondrial dynamics and cellular stress responses. Given the importance of ATP synthase in cellular energy metabolism, recombinant ATP5I proteins are being produced for structural and functional studies, aimed at elucidating its precise role in mitochondrial function and potential implications in disease pathology. Investigating this protein provides insights into the fundamental processes of energy metabolism and identifies ATP5I as a potential therapeutic target for diseases associated with mitochondrial dysfunction. Thus, the study of recombinant ATP5I is crucial for advancing our understanding of mitochondrial biology and developing novel treatment strategies.
