| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR141 |
| Uniprot No | Q7Z602 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305aa |
| 氨基酸序列 | MPGHNTSRNSSCDPIVTPHLISLYFIVLIGGLVGVISILFLLVKMNTRSVTTMAVINLVVVHSVFLLTVPFRLTYLIKKTWMFGLPFCKFVSAMLHIHMYLTFLFYVVILVTRYLIFFKCKDKVEFYRKLHAVAASAGMWTLVIVIVVPLVVSRYGIHEEYNEEHCFKFHKELAYTYVKIINYMIVIFVIAVAVILLVFQVFIIMLMVQKLRHSLLSHQEFWAQLKNLFFIGVILVCFLPYQFFRIYYLNVVTHSNACNSKVAFYNEIFLSVTAISCYDLLLFVFGGSHWFKQKIIGLWNCVLCR |
| 分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GPR141蛋白的虚构参考文献示例(仅供格式和内容参考，实际文献需通过学术数据库检索)：
---
1. **标题**：**"Heterologous Expression and Ligand Screening of Recombinant Human GPR141"**
**作者**：Zhang, L. et al.
**摘要**：研究在昆虫细胞中表达重组人GPR141蛋白，利用放射性配体结合实验筛选潜在激动剂/拮抗剂，发现化合物X表现出高亲和力，提示其可能调节下游cAMP信号通路。
2. **标题**：**"Cryo-EM Structure Determination of GPR141 in Complex with Synthetic Peptides"**
**作者**：Watanabe, K. & Müller, T.
**摘要**：通过冷冻电镜解析重组人GPR141与人工合成肽配体的复合物结构，揭示跨膜结构域中的关键结合位点，为靶向药物设计提供结构基础。
3. **标题**：**"Functional Characterization of GPR141 in Pancreatic β-Cells Using Recombinant Protein"**
**作者**：Chen, R. et al.
**摘要**：利用HEK293细胞表达重组GPR141.发现其过表达可增强β细胞的胰岛素分泌活性，暗示GPR141可能在糖代谢调节中发挥作用。
---
**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献。若需进一步协助筛选，请提供更多研究背景或关键词。
GPR141 (G protein-coupled receptor 141) is an orphan receptor belonging to the rhodopsin-like class A GPCR family, which is predominantly expressed in the central nervous system and certain peripheral tissues. Its gene is located on human chromosome 7q36.1. encoding a seven-transmembrane domain protein with a long extracellular N-terminus. Despite being classified as an orphan due to the lack of identified endogenous ligands, GPR141 shares structural homology with neuropeptide and chemokine receptors, suggesting potential roles in modulating neuronal signaling or metabolic regulation.
Recombinant human GPR141 protein is typically produced using heterologous expression systems, such as HEK293 or insect cells, to enable structural and functional studies. This engineered protein often includes tags (e.g., FLAG, His-tag) for purification and detection. Research focuses on deorphanization efforts to identify natural ligands and elucidate its physiological roles, with emerging evidence linking GPR141 to neural development, energy homeostasis, and cancer progression. Its recombinant form serves as a critical tool for ligand-binding assays, signal transduction pathway mapping (e.g., G protein coupling preferences), and drug discovery targeting neurological or metabolic disorders. Challenges remain in characterizing its activation mechanisms and validating therapeutic potential due to limited in vivo data.
×
