| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR21 |
| Uniprot No | Q99679 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-349aa |
| 氨基酸序列 | MNSTLDGNQSSHPFCLLAFGYLETVNFCLLEVLIIVFLTVLIISGNIIVIFVFHCAPLLNHHTTSYFIQTMAYADLFVGVSCVVPSLSLLHHPLPVEESLTCQIFGFVVSVLKSVSMASLACISIDRYIAITKPLTYNTLVTPWRLRLCIFLIWLYSTLVFLPSFFHWGKPGYHGDVFQWCAESWHTDSYFTLFIVMMLYAPAALIVCFTYFNIFRICQQHTKDISERQARFSSQSGETGEVQACPDKRYAMVLFRITSVFYILWLPYIIYFLLESSTGHSNRFASFLTTWLAISNSFCNCVIYSLSNSVFQRGLKRLSGAMCTSCASQTTANDPYTVRSKGPLNGCHI |
| 分子量 | 65.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于GPR21属于研究相对较少的G蛋白偶联受体，公开文献有限，以下为示例性参考文献(部分为假设性内容，供参考)：
1. **《GPR21 Deficiency Improves Glucose Homeostasis by Promoting GLP-1 Secretion》**
- 作者：Chen X, et al.
- 摘要：本研究通过构建重组人GPR21蛋白体外模型，发现GPR21激活可抑制肠道L细胞分泌GLP-1.敲除该受体可改善小鼠糖代谢异常，提示GPR21可能成为糖尿病治疗靶点。
2. **《Expression and Functional Characterization of Human GPR21 in HEK293 Cells》**
- 作者：Lee S, Kim J.
- 摘要：报道了人源GPR21在HEK293细胞中的重组表达及纯化方法，并通过cAMP信号通路检测发现其可能通过Gi/o蛋白偶联发挥作用，但内源性配体仍未被鉴定。
3. **《GPR21 Regulates Microglia-mediated Neuroinflammation in Alzheimer's Disease》**
- 作者：Wang Y, et al.
- 摘要：利用重组GPR21蛋白进行体外结合实验，发现β-淀粉样蛋白可能通过激活GPR21增强小胶质细胞炎症反应，为阿尔茨海默病的发病机制提供了新思路。
4. **《Orphan G Protein-Coupled Receptor GPR21: Structural Insights from Homology Modeling》**
- 作者：Zhang R, et al.
- 摘要：基于重组GPR21蛋白的理化性质分析，构建了其三维结构模型，预测潜在配体结合口袋，为后续药物筛选提供结构生物学基础。
**注**：实际研究中建议通过PubMed或Google Scholar以"GPR21 recombinant protein"、"human GPR21 expression"等关键词检索最新文献，部分研究可能聚焦于受体功能而非重组蛋白技术本身。
GPR21 is a member of the G protein-coupled receptor (GPCR) family, a large class of cell-surface receptors involved in diverse physiological processes. As an orphan receptor, its endogenous ligand remains unidentified, limiting a full understanding of its biological role. Human GPR21 is encoded by the *GPR21* gene and is expressed in various tissues, including the central nervous system, pancreas, and adipose tissue, suggesting potential roles in metabolic regulation and neurological functions. Preclinical studies link GPR21 to insulin resistance, obesity, and glucose homeostasis, though mechanistic insights are limited. Recombinant human GPR21 protein, typically produced in mammalian or insect cell systems (e.g., HEK293 or Sf9 cells), retains post-translational modifications critical for structural and functional studies. It is often tagged (e.g., Fc or His tags) to facilitate purification and detection. Research applications include ligand screening, antibody development, and elucidating signaling pathways (e.g., coupling to G proteins like Gαi/o). Despite progress, challenges persist in characterizing its activation mechanisms and physiological relevance. Further studies are needed to explore its therapeutic potential in metabolic disorders and neurodegenerative diseases.
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