Recombinant Human HINT3 Protein

The human HINT3 (histidine triad nucleotide-binding protein 3) belongs to the HIT superfamily, a group of conserved proteins characterized by a histidine triad (HxHxH) motif involved in nucleotide binding and hydrolysis. HINT3 is encoded by the *HINT3* gene and shares structural homology with other HINT family members, though its specific biological roles remain less defined compared to well-studied isoforms like HINT1. It is postulated to function in nucleotide metabolism, cellular signaling, or modulation of protein-protein interactions, possibly influencing processes such as apoptosis, tumor suppression, or neurological functions. Recombinant HINT3 protein is typically produced in vitro using expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical properties, enzymatic activity, and interactome. Interest in HINT3 stems from its potential regulatory roles in disease contexts; for example, dysregulation of HIT proteins has been linked to cancer and neurodegenerative disorders. However, the exact physiological substrates and pathways involving HINT3 are still under investigation. Current research focuses on elucidating its 3D structure, catalytic mechanisms, and possible therapeutic applications, such as targeting nucleotide-related pathways in drug development. Its recombinant form serves as a crucial tool for high-throughput screening, antibody production, and mechanistic studies aiming to uncover its relevance in human health and disease.