| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2BE |
| Uniprot No | P62807 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-126aa |
| 氨基酸序列 | MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK |
| 分子量 | 13.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HIST1H2BE蛋白的3篇参考文献示例，基于领域内常见研究方向整合而成：
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1. **《Expression and purification of recombinant human histone H2B subtypes for chromatin assembly studies》**
**作者**：Smith A, et al.
**摘要**：该研究描述了大肠杆菌中重组表达人组蛋白H2B亚型(包括HIST1H2BE)的方法，并通过亲和层析和离子交换层析进行纯化。纯化的蛋白用于体外核小体重建实验，验证了其在染色质结构研究中的功能性。
2. **《Epigenetic regulation mediated by histone H2B variants: Insights from in vitro reconstitution assays》**
**作者**：Zhang Y, et al.
**摘要**：文章探讨了H2B组蛋白变体(如HIST1H2BE)在表观遗传调控中的作用。研究利用重组蛋白结合体外染色质模型，揭示了H2B亚型对组蛋白修饰酶(如甲基转移酶)活性及基因沉默的影响。
3. **《HIST1H2BE phosphorylation promotes DNA damage response in human cells》**
**作者**：Tanaka R, et al.
**摘要**：通过表达磷酸化突变体的重组HIST1H2BE蛋白，研究发现其在DNA损伤修复中的关键作用。重组蛋白的体外实验表明，HIST1H2BE的特定磷酸化位点可通过调控染色质松散促进修复因子招募。
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**注意**：以上文献为示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“recombinant HIST1H2BE”“human H2B expression”)获取。部分研究可能隶属于更广泛的组蛋白重组表达体系(如核小体重建技术相关论文)。
The human HIST1H2BE protein is a member of the histone H2B family, essential for chromatin structure and gene regulation. Histones, including H2B, assemble into nucleosomes by forming an octamer core (two each of H2A, H2B, H3. H4) around which DNA wraps, enabling chromatin compaction and dynamic regulation of transcription, replication, and repair. HIST1H2BE, encoded by the HIST1H2BE gene located on chromosome 6 (6p22.2), is one of multiple H2B variants that exhibit tissue- or cell stage-specific expression, suggesting specialized roles in epigenetic modulation. Recombinant HIST1H2BE is produced using expression systems (e.g., E. coli, mammalian cells) to generate purified, tag-fused proteins for research. Its applications include studying chromatin remodeling, histone post-translational modifications (e.g., phosphorylation, ubiquitination), and interactions with DNA or regulatory proteins. Researchers also utilize it to investigate diseases linked to histone dysfunction, such as cancers and developmental disorders. Recombinant production enables controlled studies on H2B’s role in apoptosis, DNA damage response, and viral defense mechanisms. Its sequence conservation across eukaryotes underscores its fundamental biological significance, while structural studies of recombinant forms help dissect mechanistic details of nucleosome dynamics.
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