| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRMT1L2 |
| Uniprot No | Q9JIF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-353aa |
| 氨基酸序列 | MHHHHHHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGRGSHMAAAEAANCIMEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR |
| 分子量 | 84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HRMT1L2蛋白的模拟参考文献示例(注：HRMT1L2为假设蛋白名称，文献内容为虚构示例)：
1. **文献名称**：*"Recombinant Human HRMT1L2: Expression, Purification, and Enzymatic Characterization"*
**作者**：Smith A, et al.
**摘要**：本研究报道了HRMT1L2蛋白在大肠杆菌中的重组表达和亲和纯化方法，并证实其具有组蛋白H3K4特异性甲基转移酶活性，为表观遗传调控研究提供工具。
2. **文献名称**：*"HRMT1L2 Mediates DNA Damage Response via Methylation of p53"*
**作者**：Chen L, et al.
**摘要**：发现HRMT1L2通过催化肿瘤抑制蛋白p53的甲基化修饰，增强其在DNA损伤修复中的稳定性，揭示该蛋白在癌症中的潜在调控机制。
3. **文献名称**：*"Structural Insights into HRMT1L2 Catalytic Domain by Cryo-EM"*
**作者**：Yamamoto K, et al.
**摘要**：利用冷冻电镜解析HRMT1L2催化核心的三维结构，阐明其底物结合位点和甲基供体SAM的相互作用模式，为抑制剂设计提供结构基础。
4. **文献名称**：*"HRMT1L2 Knockdown Impairs Embryonic Stem Cell Differentiation"*
**作者**：Garcia R, et al.
**摘要**：通过RNA干扰技术证明HRMT1L2缺失导致胚胎干细胞分化异常，提示其在多能性维持和早期发育中的关键作用。
---
**注意**：以上文献及内容均为模拟生成，若需真实文献，建议在PubMed、Web of Science等平台检索或核实蛋白名称准确性。
**Background of Recombinant Human HRMT1L2 Protein**
HRMT1L2 (Heterogeneous Nuclear Ribonucleoprotein Methyltransferase 1-Like 2), also known as METTL23. is a member of the methyltransferase-like (METTL) protein family. It is implicated in catalyzing methyl group transfer, a post-translational modification critical for regulating protein function, RNA metabolism, and cellular signaling. HRMT1L2 contains a conserved S-adenosylmethionine (SAM)-binding domain, characteristic of methyltransferases, though its precise enzymatic targets and biological roles remain under investigation.
Studies suggest HRMT1L2 may play a role in neurodevelopment and cognitive function, as it is highly expressed in the brain. It has been linked to chromatin remodeling and transcriptional regulation, potentially influencing gene expression patterns during cellular differentiation. Additionally, dysregulation of HRMT1L2 has been associated with intellectual disability disorders, underscoring its importance in neurological health.
Recombinant HRMT1L2 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional and structural studies. Its purified form facilitates *in vitro* assays to decipher substrate specificity, methylation activity, and interactions with nucleic acids or proteins. Further research on HRMT1L2 could unveil novel mechanisms in epigenetic regulation and therapeutic strategies for neurodevelopmental disorders.
×
