| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CUZD1 |
| Uniprot No | Q86UP6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-607aa |
| 氨基酸序列 | MELVRRLMPLTLLILSCLAELTMAEAEGNASCTVSLGGANMAETHKAMILQLNPSENCTWTIERPENKSIRIIFSYVQLDPDGSCESENIKVFDGTSSNGPLLGQVCSKNDYVPVFESSSSTLTFQIVTDSARIQRTVFVFYYFFSPNISIPNCGGYLDTLEGSFTSPNYPKPHPELAYCVWHIQVEKDYKIKLNFKEIFLEIDKQCKFDFLAIYDGPSTNSGLIGQVCGRVTPTFESSSNSLTVVLSTDYANSYRGFSASYTSIYAENINTTSLTCSSDRMRVIISKSYLEAFNSNGNNLQLKDPTCRPKLSNVVEFSVPLNGCGTIRKVEDQSITYTNIITFSASSTSEVITRQKQLQIIVKCEMGHNSTVEIIYITEDDVIQSQNALGKYNTSMALFESNSFEKTILESPYYVDLNQTLFVQVSLHTSDPNLVVFLDTCRASPTSDFASPTYDLIKSGCSRDETCKVYPLFGHYGRFQFNAFKFLRSMSSVYLQCKVLICDSSDHQSRCNQGCVSRSKRDISSYKWKTDSIIGPIRLKRDRSASGNSGFQHETHAEETPNQPFNSVHLFSFMVLALNVVTVATITVRHFVNQRADYKYQKLQNY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CUZD1重组蛋白的3篇参考文献及其摘要概括：
1. **《CUZD1 is a critical mediator of the TGF-β/ETS1 signaling axis in pancreatic cancer chemoresistance》**
- 作者：Li, Y. et al.
- 摘要：研究通过重组CUZD1蛋白验证其在胰腺癌中的功能，发现其通过TGF-β/ETS1通路介导化疗耐药性，为潜在治疗靶点。
2. **《Recombinant CUZD1 protein as a novel serological biomarker for ovarian cancer detection》**
- 作者：Wang, H. et al.
- 摘要：开发重组CUZD1蛋白用于卵巢癌血清学检测，证实其高特异性及敏感性，优于传统标志物CA125.
3. **《Expression and purification of human CUZD1 in E. coli: implications for structural studies》**
- 作者：Zhang, Q. et al.
- 摘要：报道在大肠杆菌中高效表达可溶性CUZD1重组蛋白的优化策略，并解析其结构域功能，为后续机制研究提供基础。
(注：以上文献信息为示例，若需真实文献请通过学术数据库检索。)
CUZD1 (CUB and Zona Pellucida-Like Domains-Containing Protein 1), also known as UO-44 or CUBZ1. is a secreted glycoprotein encoded by the *CUZD1* gene. It belongs to the CUB domain-containing protein family, characterized by conserved structural motifs involved in protein-protein interactions. CUZD1 contains an N-terminal CUB domain and a C-terminal zona pellucida (ZP) domain, both critical for its biological functions. The protein is predominantly expressed in reproductive tissues, including the ovary, uterus, and testis, as well as in the pancreas and gastrointestinal tract.
Research suggests CUZD1 plays roles in cell adhesion, tissue differentiation, and signaling pathways. It has been implicated in reproductive processes, such as ovarian follicle development and endometrial remodeling. Notably, CUZD1 is dysregulated in several pathologies. Elevated expression has been observed in ovarian cancer, pancreatic ductal adenocarcinoma, and endometriosis, where it may contribute to tumor progression, metastasis, or inflammatory responses. Its interaction with extracellular matrix components and serine protease inhibitors (e.g., SERPINB11) highlights potential regulatory mechanisms in disease contexts.
Recombinant CUZD1 protein is engineered for in vitro and in vivo studies to elucidate its molecular functions. Produced via heterologous expression systems (e.g., mammalian or insect cells), it retains post-translational modifications critical for activity. Applications include ligand-receptor interaction assays, antibody development for diagnostic tools, and exploring its role as a biomarker or therapeutic target. However, functional redundancy with other CUB-ZP proteins and unclear signaling pathways remain challenges. Current research focuses on resolving its structure-function relationships and validating its clinical relevance in oncology and reproductive health.
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