| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BOC |
| Uniprot No | Q9BWV1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1114aa |
| 氨基酸序列 | MLRGTMTAWRGMRPEVTLACLLLATAGCFADLNEVPQVTVQPASTVQKPGGTVILGCVVEPPRMNVTWRLNGKELNGSDDALGVLITHGTLVITALNNHTVGRYQCVARMPAGAVASVPATVTLANLQDFKLDVQHVIEVDEGNTAVIACHLPESHPKAQVRYSVKQEWLEASRGNYLIMPSGNLQIVNASQEDEGMYKCAAYNPVTQEVKTSGSSDRLRVRRSTAEAARIIYPPEAQTIIVTKGQSLILECVASGIPPPRVTWAKDGSSVTGYNKTRFLLSNLLIDTTSEEDSGTYRCMADNGVGQPGAAVILYNVQVFEPPEVTMELSQLVIPWGQSAKLTCEVRGNPPPSVLWLRNAVPLISSQRLRLSRRALRVLSMGPEDEGVYQCMAENEVGSAHAVVQLRTSRPSITPRLWQDAELATGTPPVSPSKLGNPEQMLRGQPALPRPPTSVGPASPQCPGEKGQGAPAEAPIILSSPRTSKTDSYELVWRPRHEGSGRAPILYYVVKHRKVTNSSDDWTISGIPANQHRLTLTRLDPGSLYEVEMAAYNCAGEGQTAMVTFRTGRRPKPEIMASKEQQIQRDDPGASPQSSSQPDHGRLSPPEAPDRPTISTASETSVYVTWIPRGNGGFPIQSFRVEYKKLKKVGDWILATSAIPPSRLSVEITGLEKGTSYKFRVRALNMLGESEPSAPSRPYVVSGYSGRVYERPVAGPYITFTDAVNETTIMLKWMYIPASNNNTPIHGFYIYYRPTDSDNDSDYKKDMVEGDKYWHSISHLQPETSYDIKMQCFNEGGESEFSNVMICETKARKSSGQPGRLPPPTLAPPQPPLPETIERPVGTGAMVARSSDLPYLIVGVVLGSIVLIIVTFIPFCLWRAWSKQKHTTDLGFPRSALPPSCPYTMVPLGGLPGHQASGQPYLSGISGRACANGIHMNRGCPSAAVGYPGMKPQQHCPGELQQQSDTSSLLRQTHLGNGYDPQSHQITRGPKSSPDEGSFLYTLPDDSTHQLLQPHHDCCQRQEQPAAVGQSGVRRAPDSPVLEAVWDPPFHSGPPCCLGLVPVEEVDSPDSCQVSGGDWCPQHPVGAYVGQEPGMQLSPGPLVRVSFETPPLTI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BOC重组蛋白的模拟参考文献示例(注：文献信息为虚构示例，仅供格式参考，实际文献需通过学术数据库检索获取)：
1. **文献名称**：*BOC Recombinant Protein Regulates Neural Crest Cell Migration via Glypican Interaction*
**作者**：Smith J, et al.
**摘要**：研究通过表达BOC重组蛋白，揭示其通过结合细胞表面Glypican分子调控神经嵴细胞迁移的机制，为胚胎发育异常提供新见解。
2. **文献名称**：*Structural Characterization of BOC Extracellular Domain and Its Role in Hedgehog Signaling*
**作者**：Lee H, et al.
**摘要**：解析BOC重组蛋白胞外域晶体结构，证明其作为Shh共受体增强Hedgehog信号通路活性，影响干细胞分化方向。
3. **文献名称**：*BOC Recombinant Protein Modulates Cell Adhesion in Cancer Metastasis*
**作者**：Zhang R, et al.
**摘要**：体外实验表明，BOC重组蛋白通过干扰Integrin-FAK信号通路抑制肿瘤细胞黏附，提示其潜在抗转移治疗价值。
4. **文献名称**：*BOC-Fc Fusion Protein Promotes Axon Regeneration in Spinal Cord Injury Models*
**作者**：Garcia M, et al.
**摘要**：构建BOC-Fc重组融合蛋白，证实其通过激活Robo-Slit轴突导向通路促进脊髓损伤后轴突再生，具有神经修复潜力。
**建议**：实际研究中请通过PubMed、Web of Science或Google Scholar检索关键词“BOC recombinant protein”“BOC signaling”等，筛选近五年高影响力文献。
**Background of BOC Recombinant Proteins**
BOC (Brother of CDO) is a cell surface receptor belonging to the immunoglobulin (Ig) superfamily, initially identified as a homolog of the cell adhesion molecule CDO (Cell Adhesion Molecule-Related/Downregulated by Oncogenes). It plays critical roles in developmental processes, particularly in neural tube formation, neuronal migration, and axon guidance. Structurally, BOC features multiple Ig-like domains and fibronectin type III repeats, enabling interactions with extracellular ligands like Sonic Hedgehog (Shh), a key morphogen in tissue patterning and cell proliferation.
BOC functions as a co-receptor in the Shh signaling pathway, modulating signal transduction by forming complexes with the primary receptor Patched (PTCH1) and the transmembrane protein Smoothened (SMO). Dysregulation of BOC-mediated signaling has been linked to developmental disorders and cancers, underscoring its biological and therapeutic relevance.
Recombinant BOC proteins are engineered *in vitro* using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional domains of the protein. These proteins retain ligand-binding or signaling activity, enabling mechanistic studies of BOC’s role in development and disease. Researchers utilize them to investigate protein-protein interactions, pathway activation, and downstream gene regulation.
In drug discovery, BOC recombinant proteins serve as tools for high-throughput screening of molecules targeting Shh-associated pathologies, such as medulloblastoma or basal cell carcinoma. They also hold potential as therapeutic agents or biomarkers in regenerative medicine and cancer diagnostics.
Overall, BOC recombinant proteins bridge structural, functional, and translational research, offering insights into developmental biology and opportunities for therapeutic innovation. Their versatility continues to drive advances in understanding cell signaling networks and their disease-related aberrations.
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