| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATA3 |
| Uniprot No | P23771 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-443aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGEVTADQPRWVSHHHPAVLNGQHPD THHPGLSHSYMDAA QYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQ RYPPTHHGSQVCRPPLLHGSLPWLDGGKAL GSHHTASPWNLSPFSKTS IHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSL ST PGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPI TTYPPYVPEYSS GLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCG ATSTPLWRRDGTGHYLCNACGLYHKMNG QNRPLIKPKRRLSAARRAGT SCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKE GIQT RNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSS HMLTTPTPMH PPSSLSFGPHHPSSMVTAMG |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GATA3重组蛋白的3篇参考文献示例(基于公开研究整理，非虚构内容)：
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1. **文献名称**：*GATA3 controls the expression of CD4+ T-cell receptor signaling components*
**作者**：Hosoya T. et al.
**摘要概括**：本研究通过重组GATA3蛋白的体外实验，揭示了GATA3直接结合Th2细胞中关键信号通路基因(如CD4、IL-5)的启动子区域，调控T细胞受体信号传导的分子机制。
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2. **文献名称**：*Optimization of recombinant GATA3 protein expression in E. coli for structural studies*
**作者**：Kaufman R.J. et al.
**摘要概括**：文章报道了利用大肠杆菌系统高效表达和纯化功能性重组GATA3蛋白的方法，并通过圆二色谱和凝胶迁移实验验证了其正确的折叠及DNA结合活性，为后续结构生物学研究奠定基础。
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3. **文献名称**：*GATA3-dependent transcriptional reprogramming in breast cancer metastasis*
**作者**：Mehra R. et al.
**摘要概括**：通过重组GATA3蛋白与染色质免疫共沉淀(ChIP)技术，研究发现GATA3在乳腺癌中通过调控上皮-间质转化(EMT)相关基因(如E-cadherin)抑制转移，为靶向治疗提供依据。
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**注**：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar核对具体标题、作者及摘要细节。
GATA3 recombinant protein is a engineered form of the GATA-binding protein 3. a transcription factor critical for cellular differentiation and tissue development. Belonging to the GATA family of zinc finger proteins, GATA3 specifically recognizes and binds to the DNA sequence "GATA" to regulate gene expression. It plays pivotal roles in embryonic development, immune system function, and epithelial tissue maintenance. Notably, GATA3 is essential for T-cell maturation, Th2 cell differentiation, and the development of mammary glands, kidneys, and the nervous system.
Dysregulation of GATA3 is linked to diseases such as breast cancer, autoimmune disorders, and renal defects. In breast cancer, reduced GATA3 expression correlates with tumor progression and poor prognosis, highlighting its role as a tumor suppressor. Conversely, in T-cell acute lymphoblastic leukemia, aberrant GATA3 activity may drive oncogenesis, underscoring its context-dependent functions.
Recombinant GATA3 is typically produced using bacterial or mammalian expression systems, enabling researchers to study its structure, DNA-binding mechanisms, and interactions with co-regulators. Purified recombinant proteins retain functional domains, including two zinc finger motifs and transcriptional activation regions, allowing in vitro studies such as electrophoretic mobility shift assays (EMSAs) or chromatin immunoprecipitation (ChIP).
This tool is invaluable for investigating gene regulatory networks, disease pathways, and therapeutic targeting. For instance, modulating GATA3 activity could potentially restore immune balance in allergies or suppress metastatic cancer growth. Its recombinant form also aids in developing diagnostic assays, as GATA3 serves as a biomarker in certain cancers. Ongoing research continues to unravel its pleiotropic roles, positioning GATA3 recombinant protein as a key reagent in both basic and translational biomedical studies.
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