关键信息
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基因名
IMPDH2
- 应用
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别名
IMPDH2；IMPD2；Inosine-5'-monophosphate dehydrogenase 2
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种属
Human
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表达系统
E. coli
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标签
N-6His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P12268
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表达区间
1-514aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMADYLISGGTSYVPDDGLTAQQLFNCGDGL TYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAI AMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDV FEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMT KREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKN RDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNS IFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSI CITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALG ASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRY FSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVR AMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLF
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分子量
57.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, 5% Trehalose
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
IMPDH2 (Inosine Monophosphate Dehydrogenase 2) is an important enzyme that plays a critical role in the de novo synthesis of purines, specifically in the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP). This pathway is vital for nucleotide biosynthesis, affecting cell proliferation and immune response. Research has focused on IMPDH2 due to its potential as a therapeutic target in various diseases, including cancer and autoimmune disorders. In particular, selective inhibitors of IMPDH2 can selectively suppress lymphocyte proliferation, making it a promising strategy for immunosuppression in transplantation and for treating autoimmune diseases. Furthermore, the enzyme's distinct role in different tissues compared to its isoform IMPDH1 provides opportunities for the development of specific inhibitors that could minimize side effects. The production of recombinant IMPDH2 protein is essential for high-throughput screening of inhibitors and for studying the enzyme’s structure-function relationships. Understanding the mechanism of IMPDH2 and developing targeted therapeutics could pave the way for innovative treatments in oncology and immunology. Research continues to explore the biochemical characteristics of IMPDH2, its regulatory mechanisms, and its interactions with other metabolic pathways, making it a significant focus in the field of molecular biology and pharmacology.
