关键信息
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基因名
FTSJ1
- 应用
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别名
FTSJ1；AFT；FTSJD1；Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UET6
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表达区间
1-329aa
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氨基酸序列
MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLSQKIGGQGSGHVVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGAPDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQLDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKRKGQLAKEIRPQDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMSCSP
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分子量
40.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
FTSJ1, or FTSJ1 (FtsJ RNA methyltransferase 1), is a member of the FtsJ RNA methyltransferase family, which plays a significant role in the post-transcriptional modification of RNA molecules, particularly in the context of ribosomal RNA (rRNA) and messenger RNA (mRNA) processing. Research has shown that FTSJ1 is involved in various cellular processes, including RNA stability, translation regulation, and ribosome biogenesis, which are crucial for maintaining cellular homeostasis and function. Mutations in the FTSJ1 gene have been associated with X-linked intellectual disability and other neurodevelopmental disorders, highlighting its importance in brain function and development. The understanding of FTSJ1's structure and function is critical for elucidating its role in disease pathology and for potential therapeutic interventions. Recombinant FTSJ1 protein is often produced for detailed biochemical analyses, including studies on its methyltransferase activity, substrate specificity, and interaction with other cellular components. This research not only advances our knowledge of RNA metabolism but also opens avenues for targeting FTSJ1 in disorders linked to its dysfunction, making it a promising candidate for future biomedical investigations.
